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第 五 章. 微 生 物 生 物 技 術. 讀完本章節之後,讀者應該可以做到下列幾點. 可就原核細胞結構與真核細胞結構有著不同之處來舉例,並且能描述出許多常見的細菌形狀。 描述出細菌的實質特徵,並以此做為生物技術應用時有用的工具與生物模式。 可就範例說明酵母如何作為生物技術的一種有價值的角色來舉例。 定義醱酵過程,並且討論在酒精和乳酸醱酵等兩者之間的不同之處。除此之外,可就在一般食品和酒精飲料的製程中,每種醱酵過程的重要來舉例。 可就 DNA 重組的細菌,列舉出它們所產生的具有醫學重要性的蛋白質。

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第 五 章

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6428050


6428050

  • DNA


6428050

  • 5.1

  • 5.2

  • 5.3

  • 5.4

  • 5.5

  • 5.6

  • 5.7


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6428050

5.1

  • (eukaryotic)

  • (prokaryotic cells)(Domains Bacteria)(Domain Archaea)


6428050

5.1

    • DNADNA

    • DNA

    • (peptidoglycan)


6428050

5.1

  • (cocci)

  • (bacilli)

5.1


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5.1

5.2


5 1 1

5.1.1

  • (yeast)

  • (fungi)

  • (aerobic condition)

  • (anaerobic condition)

  • (batch process)


6428050

5.2

  • 5.2.1

    • (thermostable enzyme)

    • (thermophile)

  • 5.2.2

    • (transformation)

    • (competent cell)

    • (electroporation)


5 2 2

5.2.2

5.3


5 2 21

5.2.2

5.4


5 2 3

5.2.3

    • (fusion protein)

    • (expression vector)

    • (affinity chromatography)


5 2 31

5.2.3

5.5


5 2 32

5.2.3

    • (bioluminescence)

    • (luciferase)

    • (reporter gene)


5 2 33

5.2.3

5.6


5 2 34

5.2.3

5.7


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5.3

  • 5.3.1

    • (rennin)

    • (fermentation)

      • (adenosine triphosphate, ATP)

      • (aerobes)

      • (anaerobe)

      • (lactic acid fermentation)

      • ( )[alcohol (ethanol) fermentation]


5 3 1

5.3.1

5.8


5 3 2

5.3.2

    • (Type I diabetes mellitus)(insulin-dependent diabetes mellitus)

    • - [(-gal)]


5 3 21

5.3.2

5.9


5 3 22

5.3.2

5.1


5 3 3

5.3.3

  • (antibiotics)

  • (antimicrobial drug)


5 3 31

5.3.3

5.2


5 3 32

5.3.3

5.10


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5.3

  • 5.3.4

  • 5.3.5


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5.4

5.11


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5.4

  • (pathogens)

  • (vaccination)

  • DPT(DPT vaccine)

  • MMR[ (measles)(mumps)(rubella)]

  • OPV[ (oral polio vaccine)]


5 4 1

5.4.1

  • (antigens)

  • (antibody-mediated immunity)

  • B(B lymphocytes) [B(B cells)]

  • (leukocyte)

  • T(T lymphocytes) [ T(T cells)]

  • (plasma cells)

  • (macrophage)


5 4 11

5.4.1

5.12


5 4 12

5.4.1

5.13


5 4 2

5.4.2

  • (vaccines)

    • (subunit vaccines)

    • (attenuated vaccines)

    • (inactivated vaccines)

    • (retroviruses)


5 4 21

5.4.2

5.14HIV


5 4 22

5.4.2

  • (ABCD ) ( )HIV


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5.5

  • (Microbial Genome Program, MGP)

  • 5.5.1

  • 5.5.2


5 5 2

5.5.2

5.15


5 5 3

5.5.3

5.3

Sawyer T. K. Biotechniques 30(1): 2001. TIGR (www.tigr.org/tdb/mdb/mdbcomplete) GoldTM (wit.integratedgenomics.com/GOLD)


5 5 4

5.5.4

5.4

Haseltine, W. A. (2001): Scientific American, 285: 56~63


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5.6

  • 5.6.1

  • 5.6.2


5 6 1

5.6.1

5.16


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5.7

  • (bioterrorism)

  • 5.7.1

    • (bioweapons)


5 7 1

5.7.1

5.5


5 7 11

5.7.1

5.17


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5.7

  • 5.7.2

  • 5.7.3


5 7 3

5.7.3

5.18


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