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Effects of long term irrigation with reclaimed wastewater on the efficacy and fate of ALS inhibiting herbicides

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Effects of long term irrigation with reclaimed wastewater on the efficacy and fate of ALS inhibiting herbicides. Gal Dvorkin, M Manor, M Sibony, B Chefetz and B Rubin The Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University in Jerusalem, Rehovot, Israel .

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slide1

Effects of long term irrigation with reclaimed wastewater on the efficacy and fate of ALS inhibiting herbicides

Gal Dvorkin, M Manor, M Sibony, B Chefetz and B Rubin

The Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University in Jerusalem, Rehovot, Israel

slide2

Na’an- double cropping irrigated with pivot - 2.8.2006

The field is irrigated with reclaimed wastewater (RWW) for the last 40 years

introduction

applied herbicides at na ad cotton field 2006
Applied herbicides at Na’ad cotton field 2006
  • 28.4.06 – Glyphosate 0.84 kg ae/ha
  • 30.4.06 – Prometryn 1.5 kg/ha
  • 21.5.06– Trifloxysulfuron 11.25 g/ha
  • 11.6.06 – Fluometuron 1.25 kg/ha
  • 26.6.06 – Prometryn 1.4 kg/ha
  • 14.7.06 – Trifloxysulfuron 11.25 g/ha

introduction

the main problems in na an field
The main problems in Na’an field
  • The trifloxysulfuron that was applied twice in the highest recommended dose, was not able to control the Amaranthus palmeri.
  • Wheat that followed the cotton 4 months after the trifloxysulfuron application, was not damaged by residues that were supposed to remain in the soil.

introduction

hypothesis
Hypothesis
  • The long term irrigation with RWW have caused changes in the soil (OM and microorganisms), thus decreasing the persistence of ALS herbicides.
  • The Amaranthus palmeri populationdeveloped resistance to trifloxysulfuron

hypothesis

research objective
Research Objective
  • To elucidate the effect of a long term irrigation with RWW on the behavior of ALS inhibiting herbicide trifloxysulfuron in the soil
materials and methods
Materials and Methods
  • Field experiments were conducted at the problematic field, in 2007,2008 and 2009.
  • Dose response curves to trifloxysulfuron in RWW irrigated soil and in fresh water (FW) irrigated soil
  • Develop chemical analysis method to extract and estimate trifloxysulfuron residues from the soil.
the field experiments
The field experiments
  • Herbicides were sprayed with a “backpack sprayer”, each plot 2 m wide and 12 m long
  • Every 2 weeks soil samples were taken using soil driller
  • Herbicide content in the soil samples was examined using Sorghum bicolor as test plant, and harvested 3 weeks later.

Materials and methods

results field experiment 2008
Results field experiment 2008

Pelmer amaranth on the day of post-em application -16.6.08

Results

slide12

150 mm

14 day

Trifloxy 11.25

Control

460 mm

54

40

178 mm

Control

Trifloxy 11.25

Control

Trifloxy 11.25

27

Bioassay of soil samples

70 mm

0

Trifloxy 11.25

Control

Control

Trifloxy 11.25

Results

comparing the effect of trifloxysulfuron in rww soil and fw irrigated soil
Comparing the effect of trifloxysulfuron in RWW soil and FW irrigated soil
  • RWW irrigated soil was collected from Na’an field, and FW irrigated soil was collected from near by field.
  • Both soils were air dried, sieved (5 mm), and were put in 7*7*7 (cm) plastic pots.
  • The pots were planted with wheat, and sprayed with trifloxysulfuron and irrigated with FW.
  • After 3 weeks in the screen house, Plant harvested and shoot fresh wt was recorded

Materials and methods

slide17

Results

RWW soil- Trifloxysulfuron

control

1.9 g ai/ha

3.8 g ai/ha

30 g ai/ha

7.5 g ai/ha

15 g ai/ha

FW soil- Trifloxysulfuron

1.9 g ai/ha

3.8 g ai/ha

30 g ai/ha

7.5 g ai/ha

15 g ai/ha

control

slide19

RWW soil-trifloxysulfuron

30 g ai/ha

1.9 g ai/ha

3.8 g ai/ha

7.5 g ai/ha

15 g ai/ha

control

Fresh water soil- trifloxysulfuron

Results

chemical analysis of trifloxysulfuron in the soil
Chemical analysis of Trifloxysulfuron in the soil
  • Soil samples were collected from the field experiment in Na’an.
  • The samples were extracted and filtered.
  • Extracts were injected to LC/MS and a method for identifying trifloxysulfuron residues in the soil was developed.
conclusions
Conclusions
  • Trifloxysulfuron dissipates faster in RWW soil compare to FW soil.
  • Enhanced dissipation of trifloxysulfuron under field conditions is evident.
  • More experiments are required in order to determine the factors involved in the faster dissipation of trifloxysulfuron in the soil
acknowledgments
Acknowledgments
  • Prof. Tom Mueller, University of Tennessee.
  • Weed lab group: Coby Goldwasser, Hadas Rozenberg, Or Levy, Maor Mazraffi, Ella Cohen Alon Vallch and Tal Naamat.
  • Soil & Water lab- Michal Shechter, Ziva Hochman, Daniela Harush, Yaniv Olshansky and Shay Eldad.
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