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Packed Cell Volumes Total Proteins. Laboratory Procedures. PCV (Packed Cell Volume). In a CBC, we determine the number of RBC’s in several different ways. The quickest and easiest is called the microhematocrit /hematocrit, also referred to as the packed cell volume (PCV)

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Packed cell volumes total proteins

Packed Cell VolumesTotal Proteins

Laboratory Procedures


Pcv packed cell volume
PCV (Packed Cell Volume)

  • In a CBC, we determine the number of RBC’s in several different ways. The quickest and easiest is called the microhematocrit/hematocrit, also referred to as the packed cell volume (PCV)

  • The PCV will tell you if the animal is anemic or dehydrated.


Normal pcv values
Normal PCV Values

  • Canine: 37 – 55%Feline: 30 – 45%Equine: 32 – 52%Bovine: 24 – 46%



  • Blood sample should be spun in a and placed in a capillary tube.microhematocrit centrifuge for 2-5 minutes

  • Lay the tube in the centrifuge with the plugged end facing the outside of the centrifuge. Make sure that a balancing tube is placed opposite or have another sample across from yours.

  • Cells are heavier than plasma and are compacted at the end of the tube that has the clay plug.


Reading your pcv
Reading your PCV and placed in a capillary tube.


Plasma evaluation
Plasma Evaluation and placed in a capillary tube.

  • Plasma color and transparency may be helpful in determining a diagnosis and should be recorded in your findings.

  • Normal plasma is clear or a pale straw-yellow color

  • Cloudy Serum = lipemic

  • Reddish tinge = hemolyzed

  • Yellow = icteric (indicates possible liver disease)


Concentration of total protein total solids
Concentration of total protein / total solids and placed in a capillary tube.

  • Plasma protein concentrations estimated by refractometry is an important component of the CBC in all species.

  • Plasma used to determine the TP/TS is collected by breaking the hematocrit tube just above the buffy coat/plasma interface.


  • The plasma is allowed to flow onto the and placed in a capillary tube.refractometer. (Blow gently through the open end of the hematocrit tube with the broken end of the tube over the prism of your refractometer.)

  • Hold the refractometer up to the light and record your findings.

  • Make sure to wipe your refractometer after each use!


Blood films
Blood Films and placed in a capillary tube.

  • The blood film is used to perform the differential WBC count; estimate platelet numbers; and evaluate the morphological features of WBCs, RBCs and platelets.

  • Wedge smears are prepared by placing a small drop of blood on a clean glass microscope slide


Blood films1
Blood films and placed in a capillary tube.


Staining a slide
Staining a slide and placed in a capillary tube.

  • Always stain using the lightest to darkest stain.

  • Remember which side of your slide is up (clothes pins are marked “top”)

  • Rinse off from back side of slide

  • May heat fix to speed up process.


Performing the differential cell count
Performing the Differential Cell Count and placed in a capillary tube.

  • This is where the different white blood cells are tallied separately. This can be done by a blood counting machine, or by hand.

  • To manually count the different cells, first you must make a perfect slide. Stain the slide once it is dry.

  • Using a cell counter you will tally a total of 100 cells (this will make it easy to turn the numbers into a %)


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