Stem Cell in Pharmaceutical Research. Ferry Sandra. Stem Cell Characteristics. Definition of stem cells: stem cells are unspecialized cells that have two defining properties: Ability to self-regenerate/renew Ability to differentiate into different type of mature cells.
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Stem Cell in Pharmaceutical Research
Definition of stem cells: stem cells are unspecialized cells that have two defining properties:
Embryonic Stem Cells are taken from the Inner Cell Mass at the Blastocyst stage and cultured in a petri disk
Adult Stem Cells: undifferentiated cells found among differentiated cells of a specific tissue and are mostly multipotent cells.
Progenitor Cells: relatively more differentiated and more committed cells to differentiate into particular cells.
Granulocyte Colony Stimulating Factor (GCSF) or cyclophosphamide mobilization to periphery
Cord blood is collected from this umbilical vein. It contains 10 times richer than bone marrow in the proportion of stem cells.
Culture of PBMNCs using three kind of cocktail medium, M, X, E
Approximately 20-50% of Double (+) cells has been achieved from 7 days cultured PBMNC in several tested cocktail medium.
Putative morphology of EPC and EPC colony were observed~refer to published study (Bellik et al. 2005 & Hill et al. 2002)
Cells demonstrating double (+) fluorescence were identified as differentiating EPCs.
► With this technique, we could isolate CD34+ cells (hematopoietic stem cell)
Morphology of the cultured adipose stem cells at day 28 Culture (Passage 5) in EGM-2 medium~similar w/ BM derived mesenchymal stem cells
Characterization of the cultured cells