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PRACTICAL ON BLOOD PARASITES

PRACTICAL ON BLOOD PARASITES. Common methods for parasitological diagnosis of malaria. The two methods common in use : 1: Light microscopy 2: Rapid diagnostic tests (RDTs). Laboratory diagnosis of malaria. Rapid diagnostic tests detect malaria antigens. RDT procedure. RDT procedure.

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PRACTICAL ON BLOOD PARASITES

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  1. PRACTICAL ON BLOOD PARASITES

  2. Common methods for parasitological diagnosis of malaria The two methods common in use : 1: Light microscopy 2: Rapid diagnostic tests (RDTs).

  3. Laboratory diagnosis of malaria Rapid diagnostic tests detect malaria antigens

  4. RDT procedure

  5. RDT procedure

  6. RDT procedure

  7. Laboratory diagnosis of malaria Light microscopy:1: Preparing blood film

  8. Laboratory diagnosis of malaria Light microscopy:1: Preparing blood film

  9. Laboratory diagnosis of malaria Light microscopy: Thick and thin films

  10. Laboratory diagnosis of malaria CCMOVBD Plasmodium falciparum (trophozoite stage in thin smear) Plasmodium falciparum (trophozoite stage in thick smear)

  11. Trophozoites CCMOVBD CCMOVBD Schizont Gametocyte CCMOVBD CCMOVBD 16 Morphology of Malaria The Malaria Parasite Three developmental stages seen in blood films: • Trophozoite 2. Schizont 3. Gametocyte

  12. Plasmodium vivax CCMOVBD Plasmodium malariae Plasmodium ovale CCMOVBD Malaria Tutorials, Wellcome Trust 17 Morphology of Malaria Species of malaria is identified by its chracteristic microscopic appearance:

  13. CCMOVBD 18 Morphology of Malaria Plasmodium falciparum (trophozoite stage in thin smear)

  14. CCMOVBD 19 Morphology of Malaria Plasmodium falciparum (trophozoite stage in thick smear)

  15. CCMOVBD 20 Morphology of Malaria Plasmodium falciparum (characteristic banana-shaped or crescent –shaped gametocyte stage in thin smear)

  16. A 25 year-old male from India, who came 3 months ago was admitted in KKUH with a history of severe anaemia and intermittent high grade fever for the last two months not responding to antibiotics. WHAT IS THE DIAGNOSIS? Diagnosis: malaria or Plasmodium vivax

  17. A businessman who makes frequent trips to Thailand , presents with intermittent fever . WHAT IS THE DIAGNOSIS? Diagnosis: malaria or Plasmodium vivax

  18. A student in KSU who returned three weeks from vacation in Africa , he developed intermittent fever last week and lost consciousness a short time ago. WHAT IS THE DIAGNOSIS? Diagnosis: malaria or Plasmodium falciparum

  19. The patient was then treated with schizontocidalantimalarial drugs, a follow-up blood film is shown . ARE THERE ANY PARASITES? WHAT STAGE ? Plasmodium flaciparum , gametocyte stage

  20. A 7 year old child presented with anemia , hepatospenomegaly and fever .Not responding to antimalarials and antibiotics . Bone marrow smear is shown ARE THERE ANY PARASITES? WHAT STAGE ? Leishmania , amastigote stage

  21. Bone marrow aspiration Bone marrow amastigotes

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