EU-Russia Symposium on S&T Co-Operation in Biotechnology & EU-Russia Partnering Event in Biotechnology: Agrobiotechnolog

1. EU-RUSSIA SYMPOSIUM on S&T CO-OPERATION in BIOTECHNOLOGY and EU-RUSSIA PARTNERING EVENT in BIOTECHNOLOGY Agrobiotechnology: Plant Genomics and Grain Legume Crops. Prof. Noel Ellis, John Innes Centre, Norwich, UK. on behalf of the FP6 Grain Legumes Consortium

2. www.eugrainlegumes.org

3. Grain Legumes Integrated Project 1. The problem 2. The approach 3. The partnership 4. The outputs 5. Potential interactions

4. Grain Legumes Integrated Project legumes as a proportion of arable agriculture: N & S America Asia EU

5. 00/01 99/00 pea, faba, bean, lupin 90/91 rapeseed & sunflower dry forage 80/81 soybean meal & seed meat and fish meal 73/74 miscellaneous Grain Legumes Integrated Project Plant proteins in the EU: % deficit Production Use 76 03/04 77 02/03 77 01/02 74 68 62 78 81 5 10 20 protein Mt Source: UNIP

6. Grain Legumes Integrated Project The protein (and Nitrogen) economy of the EU is considerably out of balance. A substantial increase in legume protein production in the EU can redress agricultural imbalance and meet a large extant demand without a major impact on trade. To increase legume protein production we need to address the current constraints on their production and use.

7. Grain Legumes Integrated Project 1. The problem 2. The approach 3. The partnership 4. The outputs 5. Potential interactions

8. Grain Legumes Integrated Project Objective : To define the impact and potential of improved grain legumes on optimised animal feed and human food. Objective : To understand the factors affecting grain legume seed quality and use. Objective : To develop genomic and post- genomic tools needed to improve and sustain grain legume seed quality and supply. Objective : To coordinate and integrate grain legume research, to provide training in emerging technological approaches, to disseminate results, and transfer technology to industry Module 1: To identify optimal parameters for legumes in feed quality and safety Module 2: To use legumes to develop healthy and sustainable Agriculture Module 3: To investigate Variation in grain legume seed Composition Module 4: To identify the factors affecting grain legume seed composition Module 5: To develop new genetic & genomic tools for grain legume improvement Module 6: To develop new bioinformatic tools needed to improve grain legume seed quality Module 7: To provide coordination & training in grain legume research Module 8: To disseminate knowledge & transfer technology WP1.1 WP2.1 WP3.1 WP4.1 WP5.1 WP6.1 WP7.1 WP8.1 Lower Input Systems Environmental Grain Legumes in Sequencing Bioinformatics Coordination Dissemination Farming approaches to Effects on Seed Feed seedcomposition Quality WP1.2 WP2.2 WP3.2 WP4.2 WP5.2 WP7.2 WP8.2 Feed Processing & Economic & Novel approaches Pathogen Effects Mutagenesis Training Transfer Nutritional value environmental to alter seed on Seed Quality analysis composition WP4.3 WP5.3 Expression Profiling Plant Architecture WP4.4 WP5.4 Carbon/nitrogen Crop & Compar. Genomics allocation & seed quality Agronomy & end use Genetics & genomic tools Trait biology Integration

9. WP1.1 WP1.2 WP2.1 WP2.2 WP3.1 WP3.2 WP4.1 WP4.2 WP4.3 WP4.4 WP5.1 WP5.2 WP5.3 WP5.4 WP6.1 WP7.1 Genetics & genomics Trait biology WP7.2 WP8.1 WP8.2 Grain Legumes Integrated Project Distribution of funding 14.4 M€ over 48 months

10. Grain Legumes Integrated Project Objective : To define the impact and potential of improved grain legumes on optimised animal feed and human food. WP 1.1 Potential and behaviour of processed products of legume seeds. Udo Knauf , Fraunhofer IVV, D Module 1: To identify optimal parameters for legumes in feed quality and safety Module 2: To use legumes to develop healthy and sustainable Agriculture WP 1.2 Intestinal microflora, gut health and nutritional value in animals. Alfons Jansman, ID Lelystad BV, NL WP1.1 WP2.1 Lower Input Grain Legumes in Farming WP 2.1 Agronomic considerations of grain legumes in agriculture. ES Jensen, Risø, DK Feed WP1.2 WP2.2 Feed Processing & Economic & Nutritional value environmental analysis WP 2.2 Economic considerations of grain legumes in agriculture,including Life Cycle Analysis. T Nemecek, FAL, CH

11. Grain Legumes Integrated Project Objective : To understand the factors affecting grain legume seed quality and use. WP 3.1 High throughput approaches to characterise protein & metabolite amounts & composition R Thompson, INRA Dijon, F Impact of growth constraints WP 4.1 Abiotic stress Martin Crespi, CNRS Gif sur Yvette, F WP 4.2 Biotic stress Diego Rubiales, CSIC, Córdoba, E WP 4.3 Plant Architecture Francisco Madueño, CSIC Valencia, E Catherine Rameau, INRA Versailles, F Module 3: To investigate Variation in grain legume seed Composition Module 4: To identify the factors affecting grain legume seed composition WP3.1 WP4.1 Systems Environmental approaches to Effects on Seed seedcomposition Quality WP3.2 WP4.2 WP 3.2 Gene and allele discovery, including QTL approaches. C Domoney, JIC, UK Novel approaches Pathogen Effects to alter seed on Seed Quality composition WP 4.4 Control of flux from primary assimilation to seed filling. Christophe Salon, INRA Dijon, F WP4.3 Plant Architecture WP4.4 Carbon/nitrogen allocation & seed quality

12. Objective : To develop genomic and post- genomic tools needed to improve and sustain grain legume seed quality and supply. Module 5: To develop new genetic & genomic tools for grain legume improvement Module 6: To develop new bioinformatic tools needed to improve grain legume seed quality WP5.1 WP6.1 Sequencing Bioinformatics WP5.2 Mutagenesis WP5.3 Expression Profiling WP5.4 Crop & Compar. Genomics Grain Legumes Integrated Project M 5 Genomic tools J Denarié, INRA Toulouse, F WP 5.1 Genome sequencing Giles Oldroyd, JIC, Norwich, UK WP 5.2 Mutagenesis Adam Kondorosi, CNRS Gif sur Yvette, F WP 5.3 Expression profiling Helge Kuester, Bielefeld, D WP 5.4 Crop & Comparative Genomics György Kiss, Inst. Genetics, BRC Szeged & Gödöllő, Hu Medicago truncatula, Lotus japonicus, pea, chickpea, faba bean, bean, lentil, lupin, Phaseolus and clover WP 6.1 Bioinformatics Klaus Mayer, MIPS, Munich, D Sequence annotation, transcriptomic tools, comparative genomics, gemplasm analysis

13. Grain Legumes Integrated Project Objective : To coordinate and integrate grain legume research, to provide training in emerging technological approaches, to disseminate results, and transfer technology to industry WP 7.1 Coordination Noel Ellis, JIC, UK Module 7: To provide coordination & training in grain legume research Module 8: To disseminate knowledge & transfer technology WP 7.2 Short term fellowships & training workshops Ton Bisseling, WAU, Wageningen, NL WP7.1 WP8.1 Coordination Dissemination WP 8.1 Dissemination of Knowledge. A Schneider, AEP, Paris, F WP7.2 WP8.2 Training Transfer WP 8.2 Transfer and Exploitation of Results C Golstein, TTP, GLIP M 8 F Muel, UNIP, F Integration

14. Grain Legumes Integrated Project Dissemination & transfer Bioinformatics Comparative Genetics & Genomics Seed composition Crop performance Genetics Production Use Coordination & training

15. 15 10 5 25 50 75 WP No. 1.1 1.2 2.1 2.2 3.1 3.2 4.1 4.2 4.3 4.4 5.1 5.2 5.3 5.4 6.1 7.1 7.2 8.1 8.2 all % complete in the first 18 months % of total effort Grain Legumes Integrated Project 14.4 M€ award over 48 months 24.8 M€ total costs

16. Grain Legumes Integrated Project Distribution of funding by species chickpea lentil clover Phaseolus lupin faba bean pea Medicago truncatula Lotus japonicus

17. Grain Legumes Integrated Project 1. The problem 2. The approach 3. The partnership 4. The outputs 5. Potential interactions

18. Grain Legumes Integrated Project 50 Partners ~60 labs, 8 SMEs 17 Countries 10 EU 2 EEA (+ CH) 3 New Member States 1 Third country 14.4 M€ award over 48 months 24.8 M€ total costs 50% budget 10 partners Participating Laboratory Laboratory collaborating, or mentioned in the Technical Annexe

19. S Cz Po P N D Is I CH Hu B UK Aus NL DK E F Grain Legumes Integrated Project Number of participants by Country 3 New Member States 2 EEA 1 Third country

20. Grain Legumes Integrated Project 1. The problem 2. The approach 3. The partnership 4. The outputs 5. Potential interactions

21. Grain Legumes Integrated Project Comprehensive description of Grain Legume seed components their variability and impact Realistic assessment of the impact and use of Grain Legumes in animal feed Identification of genetic resources to counter the main constraints in Grain Legume production Definition of the use of Grain Legumes in crop rotations for sustainable agriculture: Life cycle assesment Integrated bioinformatics tools for Grain Legume genetics and genomics Genome sequence to anchor Grain Legume genetic maps. Mutant populations and genomic tools for systematic post genomics

22. Grain Legumes Integrated Project 1. The problem 2. The approach 3. The partnership 4. The outputs 5. Potential interactions

23. Training fellowships: Workshops and Courses: Grain Legumes Integrated Project WP 7.2 Training Mobility within the IP External connections of the IP (including TTP) 18 short term fellowships per year see www.eugrainlegumes.org for application detaills A workshop on bioinformatics (for breeders and young scientists) will be organised in months the 12-18 A course on the model legume Medicago truncatula is scheduled in the second year (month 13 to 18). An Ascochyta workshop.

24. IP TTP participants commercial breeders TTP end-users (feed companies) other projects National projects, CP in CGAIR? anyone needing access to this technology Grain Legumes Integrated Project

25. www.eugrainlegumes.org

26. 12 1 2 3 11 4 10 5 9 8 7 6 • 1 Lens culinaris (macrosperma) • 2 Lotus japonicus (Gifu) • Vicia narbonensis • 4 Lupinus mutabilis • 5 Cicer arieteneum (Kabuli) • 6 Phaseolus vulgaris • 7 Pisum sativum (rr) • 8 Lens culinaris (microsperma) • Medicago truncatula • (R108::Tnt1) • 10 Vicia faba • 11 Cicer arieteneum (Desi) • 12 Pisum sativum (RR) • Background: • faded pea standard petal (arar) Thanks to: Mike Ambrose, Andrew Davies, Julie Hofer, Trevor Wang