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ENS3320 Biochemical toxicology. Pesticides and Trace Organics. Introduction Pesticides and their toxic effects Trace Organics: chemicals and their receptors Toxic effects and risk Assessment of trace organics Electronic w astes & PBDE Concluding remarks. 1. Introduction.

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Pesticides and trace organics

ENS3320Biochemical toxicology

Pesticides and Trace Organics

Introduction

Pesticides and their toxic effects

Trace Organics: chemicals and their receptors

Toxic effects and risk Assessment of trace organics

Electronic wastes & PBDE

Concluding remarks


Pesticides and trace organics

1. Introduction

SOURCES OF TRACE ORGANICS

Combustion of organic substances; by products

Manufacturing of pollutant chemicals

Bio-magnification in food chains

Daily products: Meat (beef and fish), milk, egg.

Agent Orange & By-products of chlorine related chemicals, including pesticides.

Waste incineration, Metal processing, Fossil fuel combustion (vehicle emission).


Pops persistent organic pollutants

1.3 Introduction

POPs, persistent organic pollutants.

  • Lipid soluble, hard to remove, easy to accumulate, magnification via food chains with high concentrations in tertiary consumers.

  • Examples, polychlorinated Aromatic hydrocarbons (PAHs), Polycyclic biphenyls (PCBs), Dichloro-Diphenyl Trichloroethane (DDT), PBDE (Polybrominated Diphenyl Ether), Dioxins (TCDD) and furans.

  • Environmental estrogens, mimic estrogenic compounds in animal body, endocrine disrupters.

  • Related to gene mutations and cancer.


The unep 12 pops

Pesticides

Aldrin

Dieldrin

Endrin

Chlordane

DDT

Heptachlor

Mirex

Toxaphene

Hexachlorobenzene (HCB)

Industrial Chemicals

PCBs (Polychlorinated Biphenyls)

HCB

Unwanted By-products

PCDDs (Poly Chlorinated Dibenzo Dioxins)

PCDFs (Polychlorinated Dibenzo Furans)

PCBs

HCB

1.4Introduction

The UNEP 12 POPs


Pesticides and trace organics

1.5 Introduction

Toxicity Scale of Example Substance

LD50, mg/kg (approximate)

Substance

DEHP (plasticizer),

di(2 ethylhexyl)phthalate)

  • 100,000

  • 10,000

  • 1,000

  • 100

  • 10

  • 1

  • 0.1

  • 0.001

  • 0.00001

Practically non toxic, > 1.5 X 10 4 mg/kg

Ethanol

Sodium Chloride

Slightly toxic, 5 X 10 3 to 1.5 X 10 4mg/kg

Malathion

Chlordane

Heptachlor

Parathion

Moderate toxic, 500 to 5000 mg/kg

Tetrodotoxin

Dioxin

Very toxic, 50 to 500 mg/kg

Botulinus toxin


Pesticides and trace organics

2. Pesticides

Including herbicides, fungicides, rodenticides and substances used to control pests.

Control = intended for preventing, destroying, repelling or mitigating any pests

Usage: agricultural pesticides, public health pesticides, wood preservatives, anti-fouling paints, household products, etc

Four main types: [1] Organochlorine, [2] organophosphate, [3] carbamate and [4] pyrethroid pesticides.


2 1 organochlorine pesticide

2.1 Organochlorine pesticide

Commonly used in the past, but many have been removed from the market due to their health and environmental effects and their biomagnifiable properties (e.g. DDT and chlordane).

OCs and their metabolites resist degradation by chemical, physical or biological means.

Persistent and have half-live ranging from months to years and in some cases decades.

DDT is the most notorious organochlorine (OC) insecticide widely used in agricultural production around the world even today.


General effects of organochlorine pesticides

General effects of organochlorine pesticides

toxic to humans and other animals and are highly toxic to most aquatic life. They can have serious short-term and long-term impacts at low concentrations.

sub-lethal effects such as immune system and reproductive damage of some of these pesticides may also be significant

build up in the fatty tissues of humans, plants and animals. Most of them are attracted to fatty tissues and organs and are accumulated significantly through food-chain (bio-magnification) and hence large animals in high trophic levels accumulate most.


Ddt dichloro diphenyl trichloroethane

DDT (Dichloro diphenyl trichloroethane)

http://en.wikipedia.org/wiki/Rachel_Carson

Raised public awareness about the dangers of pesticides, with a specific focus on persistent OCs and DDT due to the publication of Silent Spring by Rachel Carson (1907-1964), leading to the establishment of USEPA.

Banned in many countries in the 1970s in response to public concern and mounting scientific evidence linking DDT with damage to wildlife.


Ddt d ichloro d iphenyl t richloroethane

DDT(dichlorodiphenyltrichloroethane)

  • Banned in the United States in 1972, but is now being used in many countries including China to kill mosquitoes to avoid malaria.

  • DDT is an organochlorine slowly transformed to 1,1-dichloro-2,2-bis(p-dichlorodiphenyl)ethylene (DDE) and 1,1-dichloro-2,2-bis(p-chlorophenyl)ethane (DDD), and ultimately transformed into bis(dichlorodiphenyl) acetic acid (DDA) at a very slow rate.

  • DDA are readily excreted in urine, but DDE tends to bio-concentrate and accumulate in food webs.

  • Fish, milk contaminated with DDT or metabolites DDE are major sources for human uptake.


Toxic effects of ddt

Toxic effects of DDT

  • Toxic to many types of aquatic organisms, even at low concentrations. It has moderately to slightly toxic to studied mammalian species via the oral route.

  • Chronic effects on the nervous system, liver, kidneys, and immune systems in experimental animals.

  • Adverse effects on the liver, kidney and immune system due to DDT exposure have not been demonstrated in humans.

  • Evidence for mutagenicity and genotoxicity is contradictory, but carcinogenicity is equivocal.

  • Confirmed to be environmental estrogen.


Examples of organochlorine pesticides

Examples of Organochlorine Pesticides

1) DDT: DichloroDiphenyllTrichloroethane

2) Endosulfan

3) Methoxychlor

4) Heptachlor


Methoxychlor mxc

Methoxychlor (MXC)

  • Protect crops, ornamentals, livestock, and pets against fleas, mosquitoes, cockroaches, and other insects, as a replacement for DDT

  • Readily metabolized, non-accumulative

  • No acute or chronic effects has been reported on human

  • Used extensively to protect crops, ornamentals, livestock, and pets against fleas, mosquitoes, cockroaches, and other insects,

  • Xenoestrogen and endocrine disruptor


Endosulfan and its toxic effects

Endosulfan and its toxic effects

  • USEPA Category Ib – Highly Hazardous

  • Beta- (very persistence, half life = 800 days) and alpha- isomer

  • Acutely neuro-toxic to both insects and mammals and affects CNS: Epilepsy

  • Hyperactivity, tremors, convulsions, lack of coordination, difficulty breathing, nausea and vomiting, diarrhea, lethal effects

  • Chronic endocrine disruptor: Hypothyroidism

  • Environmental estrogen

  • Reproductive and developmental toxicity


2 2 organophosphate pesticides

2.2 Organophosphate Pesticides

Most OPs are insecticides.

Developed during the early 19th century, but their effects on insects, which are similar to their effects on humans, were discovered in 1932.

Not persistent in the environment. They degrade rapidly by hydrolysis on exposure to sunlight, air, and soil.

An attractive alternative to the persistent organochlorine pesticides, such as DDT and aldrin


General effects of organophosphate

General effects of Organophosphate

Organophosphate pesticides irreversibly inactivate acetylcholinesterase, which is essential to nerve function in insects, humans, and many other animals.

Many organophosphates, in particular Chlorpyrifos,are highly toxic to fish. It is necessary to exercise extreme care and caution not to contaminate fish bearing water bodies.


Examples of organophosphate pesticides

Examples of organophosphate pesticides

Chlorpyrifos

Malathion

Dichlorvos

Parathion


Malathion cas121 75 5

Dimethoxyphosphinothioyl)thio)butanedioic acid

Ref: PAN Pesticide Database

Malathion CAS121-75-5

  • The most commonly used insecticide

    • EPA estimates that annual use of it is over 30 million pounds

  • Inhibits a CNS enzyme called acetylcholinesterase (AChE)

  • Cause over-excitation of synaptic neuron

  • Confirmed mutagen, suspected carcinogen

  • Other effects: immune system, sperm activity and hormone system


2 3 carbamate pesticides

2.3 Carbamate Pesticides

Carbamates are effective insecticides by virtue of their ability to inhibit acetylcholinesterase (AChE) in the nervous system.

The carbamylation of the enzyme is unstable, and the regeneration of AChE is relatively rapid compared with that from a phosphorylated enzyme.

They are less dangerous with regard to human exposure than organophosphorus pesticides.


Examples of carbamate pesticides

Examples of Carbamate Pesticides

Aldicarb

Carbofuran

Acute intoxication symptoms:dizziness, sweating, laboured breathing, unconsciousness, vomiting, pupillary constriction, muscle cramp, excessive salivation

Carbaryl


Aldicarb cas116 06 3

Aldicarb, 2-methyl-2-(methylthio)-propionaldehyde O-methylcarbamoyloxime

Ref: PAN Pesticide Database

Aldicarb CAS116-06-3

  • Control mites, nematodes and aphids by applying directly to the soil

    • USEPA estimated that 5.2-5.6 million pounds of aldicarb were used nationwide

  • Reversibly inactivate acetylcholinesterase

  • Highly toxic insecticide (more potent than malathion)

  • Little evidence on mutagenicity / carcinogenicity

  • Suppress immune system


2 4 pyrethroid pesticides

2.4 Pyrethroid Pesticides

Natural Pyrethrins is a botanical insecticide made from Chrysanthemum flowers while Pyrethroids such as Cypermethrin and tetramethrin are its synthetic chemical analogues.

Provide rapid knockdown effects on insects but with only little residual activity.

To increase their effectiveness, a synergist - Piperonyl butoxide (PBO) is often added to the formulations.

Toxic to fish and other aquaticorganisms, they must be used carefully so as not to contaminate the waterbodies.


Examples of pyrethroid pesticides

Examples of Pyrethroid Pesticides

Cypermethrin

Tetramethrin

Allethrin


2 5 take home exercises

2.5 Take home exercises

  • Make a table to summarize the types of chemicals, general properties of pesticides and their biological targets.

  • Compare and explain the chemical structures and toxic targets of OPs and OCs.

  • Are carbamates safer than Ops and OCs? Why?


Polychlorinated aromatic hydrocarbons pahs

3.Trace Organics: chemicals and their receptors

Polychlorinated aromatic hydrocarbons, PAHs

  • Products of tobacco smoke and combustion of fossil fuels

  • Benzo-a-pyrene as a representative chemical

  • They undergo primary (Phase I) and secondary (Phase II) detoxification steps and metabolism

  • Could become DNA adducts causing DNA damage and point mutation

  • As chemical carcinogens, leading to mutation of oncogenes and cancer.


Dioxins

3.Trace Organics: chemicals and their receptors

Dioxins

  • Polychlorinated dibenzo-p-dioxins form a group of 75 congeners, including the most toxic compound TCDD, 2,3,7,8- tetrachloro dibenzo-p-dioxin. EPA limit in drinking water is 0.00003 mg/L (ppm).

  • FDA limits for seafood: 50 ppt.

  • Waste water discharge limit: 0.5 ppt.

  • Air: 4-5 ppt or 45 ppt , related to climatic conditions.

  • US daily intake (estimated): 20 X 10 –12 g/kg bw. WHO data: 1 – 4 pg/kg/day (tolerable daily intake).


Dibenzo para dioxin structure formula and numbers of chlorinated isomers

o

Cl x

Cl y

o

o

Cl

Cl

o

Cl

Cl

2,3,7,8-tetrachlorodibenzo-p-dioxin (2,3,7,8-TCDD)

The most toxic dioxin !

3.Trace Organics: chemicals and their receptors

Dibenzo-para-dioxin structure formula and numbers of chlorinated isomers

No. of chlorines (x+y) No. of isomers

1 2

210

314

422

514

610

7 2

8 1

Total75

Adapted from IARC et al, 1997


Pesticides and trace organics

3.Trace Organics: chemicals and their receptors

A wider group of related compounds considered as dioxin-like: PCDFs (polychlorinated dibenzo furans), PCBs (polychlorinated biphenyls), diphenyl ethers, naphthalenes, etc.

1

9

O

3’

3

2

2’

2

8

4

4’

7

3

Cly

Clx

O

Clx

Cly

4

6

5

6

6’

5’

Polychlorinated dibenzodioxins

(PCDDs)

Polychlorinated biphenyls (PCBs)

9

1

2

8

3

7

O

Clx

Cly

4

6

Polychlorinated dibenzofurans (PCDFs)

Benzo[a]pyrene


Pesticides and trace organics

3. Trace Organics: chemicals and their receptors

1

9

O

Cl

Cl

2

8

7

3

Cl

O

Cl

6

Benzo-[a]-pyrene

4

TCDD, 2,3,7,8 Tetrachloride Dibenzo Dioxin

NH

3-methylcholanthrene

NH

Indolo[3,2-b]carbazole found in plants and diets

CH3


Characteristics of dioxins

3.Trace Organics: chemicals and their receptors

Characteristics of dioxins

  • Low vapor pressure and water solubility; lipid soluble

  • Persistent, chemically stable

  • Bio-accumulated in lipid, highly lipophilic

  • long half-life (5-10 years in human)

  • high affinity on Aryl hydrocarbon receptor (AhR)


Mechanism of intracellular actions of dioxins and dioxin like compounds

Mechanism of intracellular actions of dioxins and dioxin-like compounds

3.Trace Organics: chemicals and their receptors

n: nuclear pore factors

p23

Hsp90

n

dioxin

unoccupied

AHR

Ah receptor

CYP1A1

Hsp90

ER

n

CYP1A

gene induction

ARNT

AHR

AAA

AAA

heterodimer

n

AHR

AHR

DRE

n

ARNT

ARNT

Cytosol

Nucleus

CYP 450


Pesticides and trace organics

3.Trace Organics: chemicals and their receptors

Anti-estrogenic effects of TCDD

  • It was found that testosterone (a male hormone) levels DECREASED in small numbers of workers occupationally exposed to TCDD with serum TCDD concentrations of 20 pg/g blood lipid (Egeland et al., 1994).

  • Speculative of lower sperm counts in European countries to be related to higher dioxin levels.

  • Lower T4:thyroxine binding globulin ratio and plasma thyroid stimulating hormone levels related to high PCDD and TEQ.


Estrogen receptors

3.Trace Organics: chemicals and their receptors

Estrogen Receptors

  • Environmental estrogens stir debate.

  • Would chemical compounds being modified in living organisms become estrogen receptors mimics?

  • Would those chemical compounds, when accumulated, cause deficiency in reproductive system in making estrogenic effects in males (lower sperm count) or lower the estrogenicity in females (reduced egg production)?

  • Evidence found in mice, marine organisms and fish. How about human beings? Ans: Not fully understood.


Proved xenoestrogen

3.Trace Organics: chemicals and their receptors

Proved Xenoestrogen

  • Chlorinated organic compounds, e.g. Atrazine, Chlordane, DDT, Endosulfan, Methoxychlor, PCBs.

  • Plastics, e.g. Bisphenol A, nonylphenol (plastic softener).

  • Pharmaceuticals, e.g. Cimetidine (dopamine receptor blocker for ulcer treatment), synthetic estrogens (for birth control).

  • Aromatic hydrocarbons (from vehicle exhaust).


Pesticides and trace organics

3.Trace Organics: chemicals and their receptors

Environmental Estrogens!?

Estrogens

PCBs


Pesticides and trace organics

3. Trace Organics: chemicals and their receptors

Adapted from Kuiper et al., 1998. Sci. & Med. 5(4):36-45.


Pesticides and trace organics

3.Trace Organics: chemicals and their receptors

Inactive Receptor

Active Receptor

Estrogens

Anti-estrogens

Hormone Binding Region

DNA Binding Region

Other estrogenic compound

Target Gene


Pesticides and trace organics

Adapted from Kuiper et al., 1998. Sci. & Med. 5(4):36-45.

Tamoxifen: a partial anti-estrogen in use as a breast cancer therapeutic

3.Trace Organics: chemicals and their receptors


Pesticides and trace organics

Environmental estrogens are able to bind to estrogen receptors as mimic to enhance or reduce (anti-estrogenic) the activation of target genes.

ERs forming homo- (αα or ββ) heterodimers (αβ)

Other estrogenic compound

Target Gene

Estrogen, e.g. 17β estradiol.

3. Trace Organics: chemicals and their receptors


Pesticides and trace organics

Compound A

Receptor

for A

e.g. AhR

metabolised to

B-like compound

Inhibits?

Enhance ?

Receptor

for B

e.g. ER

Compound B

Q: Compare and contrast the structure and function of ER and AhR.

Explain why some dioxin and PCBs are ligands of AhR, but could also be considered as an environmental estrogens.


Pesticides and trace organics

Adapted from:

Ruegg et al., 2008. The transcription factor aryl hydrocarbon receptor nuclear translocator functions as an estrogen β-selective co-activator, and its recruitment to alternative pathways mediates anti-estrogenic effects of dioxin.Mol. Endocrinol. 22:304-316.

Adapted from:

Matthews et al., 2005. Aryl hydrocarbon receptor-mediated transcription: ligand- dependent recruitment of estrogen receptor α to 2,3,7,8-tetrachlorodibenzo-p-dioxin-responsive promoters.Mol. Cell. Biol. 25:5317-5328.


Pesticides and trace organics

4.Toxic effects and risk Assessment of trace organics

  • Dioxin has different toxicities on animals.

  • Sub-lethal effects are of major concern.

  • What mechanism of dioxins would take to cause their toxic effects?

  • How dioxins affect cell at the molecular level?

  • What component of cell can recognize dioxins?

  • It the most toxic compound known to human, what can we do to assess our risk to this compound?


Acute toxicity of tcdd species specificities

4.Toxic effects and risk Assessment of trace organics

Acute Toxicity of TCDD: species specificities

Adapted from Tschirley et, 1986


Dioxins chloracne

4.Toxic effects and risk Assessment of trace organics

DIOXINS & Chloracne

  • 1899, chloracne

  • Agent orange used in Vietnam war

  • Users and victims suffered from chloracne

  • PCBs and dioxins caused chloracne

  • Mechanisms not known, believed to be related to the halogens.

  • Causing dilations of the infundibular or suprafollicular area of the hair follicle with hyperkeratinization (accumulation of keratin and sebaceous gland secretion)


Pesticides and trace organics

4.Toxic effects and risk Assessment of trace organics

Chloracne is an occupational dermatosis with numerous acne form lesions located on the face and neck behind the ear.

Follicular Hyperkeratosis: mouse skin treated with tetrachlorobiphenyl (Puhvel et al., 1982)


Pesticides and trace organics

4.Toxic effects and risk Assessment of trace organics

Ukraine President Yushchenko was suffering from a Dioxin intoxication during his election, high concentration of TCDD (100,000 picograms (10-12 μg) per L (so 100 ppb)) was found in his blood, normal person could only have a few ppt.

photo taken on July 6 (left) and Dec 10, 2004 showed his face with symptoms of chloracne.


Other dioxin contamination accidents happened worldwide

4.Toxic effects and risk Assessment of trace organics

Other Dioxin Contamination Accidents Happened Worldwide

  • 1962-1970Agent Orange used in Vietnam war

  • 1968“Yusho”, Japan

  • 1976Accident in Seveso, Italy

  • 1979“Yucheng”, Taiwan

Adapted from Schecter et al, 1999


The yucheng incident

4.Toxic effects and risk Assessment of trace organics

The “Yucheng”(油症) Incident

Adapted from Guo, Yu, Hsu & Rogan, 1999. Environmental Health Perspective 107:715-719.

Background:

  • Central Taiwan in 1979, dioxins and PCBs mixture were used in rice oil processing.

  • About 2,000 people were found to be contaminated and mass poisoning occurred.

  • Symptoms included chloracne, hyperpigmentation, and peripheral neuropathy, and the illness was referred to as “Yucheng”(油症).

  • “Yucheng cohort” were estimated to have consumed about 1g of PCBs and 3.8 mg of PCDFs during that time!

  • They were interviewed and their clinical symptoms had been detected since then.

  • Women have higher prevalence of anemia, headache, goiter, gum pigmentation, chloracne.


Pesticides and trace organics

4.Toxic effects and risk Assessment of trace organics

Prevalence (%) of reported diseases ever diagnosed by a physician in Yucheng and control groups in Taiwan, 1993

Adapted from Guo, Yu, Hsu & Rogan, 1999. Environmental Health Perspective 107:715-719.


Basic concepts of toxic equivalency factor tef

4.Toxic effects and risk Assessment of trace organics

Basic Concepts of Toxic Equivalency Factor (TEF)

  • dioxin-like compounds exist in environmental and biological sample as complex mixtures of congeners.

  • compare toxicology of various dioxin-like compound.

  • simplify risk assessment and regulatory control.

  • Toxic Equivalency Factor (TEF) of 2,3,7,8-TCDD is 1

  • Combine PCB and dioxins together : 4 x 10 –12 g TEQ per kg bw. (TEQ = Toxic Equivalency Quotient)


Pesticides and trace organics

4.Toxic effects and risk Assessment of trace organics

Examples:

Adapted from Van den Berg et al, 1998


Pesticides and trace organics

4.Toxic effects and risk Assessment of trace organics

TEQ = (PCDDi × TEFi) + (PCDFi × TEFi) + (PCBi × TEFi)

  • The criteria for including a compound in the TEF scheme for dioxin-like compounds:

  • Show a structural relationship to the PCDDs and PCDFs

  • Bind to the Ah receptor (Ah R)

  • Elicit Ah R-mediated biochemical and toxic responses

  • Be persistent and accumulate in the food chain

Adapted from WHO et al, 1998


Pesticides and trace organics

4.Toxic effects and risk Assessment of trace organics

PCDD/PCDF levels in the general population 1980-1991

Adapted from Schecter, A (ed). 1994. Dioxins and Health. Plenum Press, New York, 710p.


Pesticides and trace organics

4.Toxic effects and risk Assessment of trace organics

Estimated Dietary Intake of PCDDs and PCDFs in the UK.


Pesticides and trace organics

4.Toxic effects and risk Assessment of trace organics

Estimated Dietary Intake of PCDDs and PCDFs for Canadian Adults as Calculated by Birmingham et al.

Adapted from Schecter, A (ed). 1994. Dioxins and Health. Plenum Press, New York, 710p.


Problems with i tef

4.Toxic effects and risk Assessment of trace organics

Problems with I-TEF

  • Tolerable Daily Intake (TDI) of 1 to 4 pg I-TEQ per kg body weight per day has been established for dioxins by the World Health Organization (WHO). The upper limit of 4 is provisional and the ultimate goal is to reduce human intake levels below 1 pg I-TEQ per kg body weight per day.

  • The TEF concept remains the most feasible approach for risk assessment purposes.

  • Pharmacokinetic differences between species (human = mouse?) can significantly influence the TEF value.

  • Unknown additive or non-additive interactions might hamper the derivation of consensus TEF values.


Pesticides and trace organics

4.Toxic effects and risk Assessment of trace organics 4

PCBs

Clx

Cly

  • PCBs are known to cause birth defects, neurological damage and thyroid imbalances. Their use was banned in the U.S. in 1976.

  • Would people at risk consuming the contaminated health food products?

  • PCBs contain 209 congeners with varying harmful effects.

  • RfDose for Aroclor 1016 is 0.00007 mg/kg/day

  • PCB is a class B2 carcinogen, the ED10 is 50 mg/kg/day


Effects of pcbs

4.Toxic effects and risk Assessment of trace organics

Effects of PCBs

  • Some PCBs are also AhR agonists with much lower TEF except PCB126 (3,3’,4,4’,5-pentaCB is 0.1) and PCB129 (3,3’,4,4’,5,5’-hexaCB is 0.01).

  • They induce CYP enzymes (2A1 and 2B1).

  • They enter the cell freely to alter calcium ion signaling in lymphocytes, neurons and muscle.

  • They produce immuno-modulatory effects, and the development of cardiovascular disease.

  • Some are even estrogenic and some are anti-estrogenic effects.


Pesticides and trace organics

5. Electronic wastes: PBDEs and metal ions

  • Directive Of The European Parliament And Of The Council On The RESTRICTION OF THE USE OF CERTAIN HAZARDOUS SUBSTANCES IN ELECTRICAL AND ELECTRONIC EQUIPMENT-(Amended by 2005/618/EC, 2005/717/EC, and 2005/747/EC)The six hazardous substances being banned are the heavy metals lead, cadmium, mercury and hexavalent chromium, as well as two groups of brominated flame retardants - polybrominated biphenyls (PBB) and polybrominated diphenyl ethers (PBDE).

  • Scientists link depressed immune systems and fewer cubs to PCB contamination of polar bears. PBDEs - suspected of having same toxic effects as PCBs. Penta bromo diphenyl ether (pentaBDE) is found in sewage sludge spread in US.


Pesticides and trace organics

5. Electronic wastes: PBDEs and metal ions

Adapted from Hooper and McDonald (2000) The PBDEs: An Emerging Environmental Challenge and Another Reason for Breast-Milk Monitoring Programs. Environ. Health Perspect. 108: 387-392.


Pesticides and trace organics

5. Electronic wastes: PBDEsand metal ions

Structures of PBDEs

BDE47: 2,2’,4,4’

BDE99: 2,2’,4,4’, 5

BDE153: 2,2’,4,4’, 5,5’

PBDEs

O

(X + Y = 10)

Brx

Bry

Thyroxine

I

I

CH2CH(NH2) COOH

O

HO

I

I

The chemical structure of BDEs also resembles thyroxine, a thyroid hormone. Initial studies indicate that BDEs could interfere with the metabolism of thyroid hormones, and with their transport throughout the body.


Pesticides and trace organics

5. Electronic wastes: PBDEs and metal ions

Bioaccumulation of PBDEs

  • PBDE levels in breast milk of North American women have increased from two to 200 parts per billion over the last decade and 300-400 times in Canadian lake trout from 1978 to 1998.

  • One of the major concerns about BDEs in breast milk is the exposure of infants, they may have neurological effects and affect IQs of new born babies.

  • Developmental exposure to brominated diphenyl ethers results in thyroid hormone disruption in rats (Zhou et al., 2002; Hallgren and Darnerud, 2002).


Toxic effects of pbdes

5. Electronic wastes: PBDEs and metal ions

Toxic effects of PBDEs

  • Serum T4 levels (thyroxine) reduced, but EROD (ethoxy-resorufin-O-deethylase) and UDPGT (uridine diphosphate glucuronosyl transferase) levels higher in exposed group.

  • T4 glucuronidation by phase II UDPGT in liver might be one of the mechanisms for lower T4 in blood, causing hypothyroidism.

  • Hypothyroxinemia during fetal or postnatal periods occur even when T3 (triiodothyronine) is normal, causing permanent functional abnormalities and mental retardation in children.


Pesticides and trace organics

6. Concluding Remarks

  • Persistent Organic Pollutants (mainly from foodstuffs, e.g. fish) cause chronic effects on human as hormone disrupters, can affect our immune system, and may even be carcinogenic.

  • Tight regulations have been imposed on them but some dangerous pesticides, including DDTs, and still being used in developing countries.

  • Due to the binding to AhR and power to induce CYP1A, TEF values have been assigned to dioxin and dioxin-like compounds. Using TEQ values, we can better manage the risk of these POPs.

  • PBDE is found in e-waste, it could affect our thyroid hormone system and may have significant impacts on growth and developments in infants.


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