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Quorum Sensing

Quorum Sensing. Sender. methionine. LuxR. metK. LuxR. GFP. pTet. S-adenosyl methionine. pTet. pLux. LuxI. 3OC6HSL(AHL). Receiver. LuxR. Quorum Sensing. Sender. methionine. LuxI. metK. LuxR. GFP. pTet. S-adenosyl methionine. pTet. pLux. AHL. LuxI. 3OC6HSL(AHL). LuxR.

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Quorum Sensing

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  1. Quorum Sensing Sender methionine LuxR metK LuxR GFP pTet S-adenosyl methionine pTet pLux LuxI 3OC6HSL(AHL) Receiver LuxR

  2. Quorum Sensing Sender methionine LuxI metK LuxR GFP pTet S-adenosyl methionine pTet pLux AHL LuxI 3OC6HSL(AHL) LuxR

  3. Quorum Sensing Sender methionine LuxI metK LuxR GFP pTet S-adenosyl methionine pTet pLux AHL LuxI 3OC6HSL(AHL) LuxR

  4. Quorum Sensing Sender methionine LuxI metK LuxR GFP pTet S-adenosyl methionine pTet pLux AHL LuxI 3OC6HSL(AHL) LuxR

  5. AHL quencher ～aiiA～ LuxI Sender pTet AHL aiiA aiiA P cons. Lacton circle

  6. AHL quencher ～aiiA～ LuxI Sender pTet AHL aiiA aiiA P cons. Lacton circle

  7. aiiAinverter Receiver LuxR GFP aiiA pTet pLux cI inverter Low AHL conc. High AHL conc.

  8. Senderの数とGFP発現 Sender Receiver LuxI LuxR GFP pTet pTet pLux Sender(BW25113 or XL10G), Methionine (10mM or 0mM) Sender:5×10^8～5×10 Receiver

  9. Senderの数とGFP発現 methionine metK S-adenosyl methionine LuxI Sender(BW25113 or XL10G), Methionine (10mM or 0mM) 3OC6HSL(AHL) Sender:5×10^8～5×10 Receiver

  10. AHL synthesis AHL synthesis methionine S-adenosyl methionine 3OC6HSL metK LuxI, acyl-ACP Sender(BW25113 or XL10G), Methionine (10mM or 0mM) Sender:5×10^8～5×10 Receiver

  11. Quorum Sensing System LuxI and LuxR aiiA SenderとReceiverの選択ができるようにする Sender Receiver Sensitive Normal aiiA Receiver Strong(Super Sender) Normal

  12. Quorum Sensing System LuxI and LuxR aiiA SenderとReceiverの選択ができるようにする Sender Receiver Sensitive Normal aiiA Receiver Strong(Super Sender) Normal

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