Announcements
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Announcements. Exam 1 is on Monday Multiple choice: 15-20 questions Matching: 8-10 questions Problems: 4-6 multipart problems (probably ~50% of total points) Multiple choice/matching problems from lecture, key terms/concepts, and homeworks (covers reading up through Chapter 5)

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Announcements

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Announcements

Announcements

Exam 1 is on Monday

Multiple choice: 15-20 questions

Matching: 8-10 questions

Problems: 4-6 multipart problems (probably ~50% of total points)

Multiple choice/matching problems from lecture, key terms/concepts, and homeworks (covers reading up through Chapter 5)

Problems similar (NOT IDENTICAL) to Homeworks 1-3

Homework keys and term lists are all on the course website

Bring a #2 pencil!

You may use a regular calculator, but no phones allowed


Bio 304 lecture 9

BIO 304 Lecture 9


Announcements

At which stage of meiosis does crossing over occur?

✓1.Prophase I

2.Prophase II

3.Metaphase I

4.Metaphase II

5.Telophase I

6.Telophase II


Single and double crossovers

Single and double crossovers


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If a dihybrid test cross produces 30% recombinant progeny, EACH parental class must represent ________% of the progeny.

1.15

2.30

✓3.35

4.50

5.70


The three point test cross

The three-point test cross:

PURPOSE:

PROCEDURE:


Example 3 point test cross

Example 3-point test cross:

3 Drosophila alleles (autosomal):

eye color: v (vermillion) or v+ (wild type, red)

wing veins: cv (no cross vein) or cv+ (wild type)

wing edges: ct (cut) or ct+ (wild type)

What is the order of the three genes and the distances between them?

Pv+ /v+cv /cv ct/ct X v/vcv+/cv+ ct+ /ct+

F1 trihybridv+ /vcv+ /cv ct+ /ct

TEST CROSS:v+ /vcv+ /cv ct+ /ctX v/vcv/cv ct/ct

F1 trihybrid femaletester male


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F2 progeny:


Step 1 identify non crossover classes

Step 1: identify non-crossover classes:


Step 2 identify double crossover classes

Step 2: identify double crossover classes


Step 3 calculate recombinant frequencies

Step 3: calculate recombinant frequencies:


Recombinant frequency for v and cv

Recombinant frequency for v and cv


Recombinant frequency for v and ct

Recombinant frequency for v and ct


Recombinant frequency for c v and ct

Recombinant frequency for cv and ct


Step 4 construct the map

Step 4: construct the map

Recombination frequency v and cv: 18.5

Recombination frequency v and ct: 13.2

Recombination frequency cv and ct: 6.4


Short cut using double recombinants to deduce gene order

Short cut: using double recombinants to deduce gene order

Double recombinants have the middle gene“flipped” relative to parental arrangement:

parentals:vcv+ ct+

v+cv ct

DCO’s:vcv+ ct

v+cv ct+


Announcements

How confident do you feel about your mastery of the material for the exam?

1.Very confident

2.I understand almost everything, but need to go over a few topics

3.I'm sure I'll understand everything once I cram this weekend

4.Not very confident


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