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Fusion cell gene expression profile

Identify dys fusion cell enhancer. Fusion cell gene expression profile. dys-gal4::UAS-CD8-GFP. btl-gal4::UAS-CD8-GFP. Identify dys target gene. dys-gal4::UAS-CD8-GFP. dys-gal4::UAS-CD8-GFP ; dys/dys. Molecular mechanism of target gene regulation by Dys::Tgo.

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Fusion cell gene expression profile

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  1. Identify dys fusion cell enhancer • Fusion cell gene expression profile dys-gal4::UAS-CD8-GFP btl-gal4::UAS-CD8-GFP • Identify dys target gene dys-gal4::UAS-CD8-GFP dys-gal4::UAS-CD8-GFP ; dys/dys • Molecular mechanism of target gene regulation by Dys::Tgo • Identify enhancer of dys targets: CG13196, CG15252 and mbo • Co-activator or repressor of Dys::Tgo?

  2. Identify dys enhancer Exon1 2 3 4 5 6 7 8 9 10 LP05454 dys 2 (1.7kb) dys intron 2a (3.8kb) dys1 (2.7kb) dys intron2b (3.8kb) dys intron1 (1.4kb) dys un (3.8kb) dys1a (1.7kb) dys1b (1.0kb) dys1b-A (500bp) dys1b-B (500bp)

  3. Enhancer for brain, hindgut and anal pad dys intron-1 dys intron-2b dys intron-2a

  4. dys1 fragment contains dys fusion cell enhancer dys un (S15-17) dys2 ( S15-17) DB DT DT LT S16 S17 dys1 (S12-17) Leading edge? DB DT LT S14 S14

  5. dys1b (S12-17) dys1a (S14-17) S12 S12 S14 S14 S16 S15

  6. dys target gene: CG15252 TCGTG TCGTG TCGTG Exon1 CG15252 TCGTG (0.8 kb) CG15252 con (3.2kb) 9.0 kB 5’ upstream

  7. CG15252 enhancer CG15252 con DT CG15252 TCGTG S16

  8. CG15252 enhancer TCGTG-d 792 bp TCGTG-b 360 ssbp TCGTG-a 185 bp TCGTG-c 774 bp 818bp TCGTG a+b (270bp) TCGTG c+d (100bp) ( NO?) (Yes!) mutation: TCGTG to GATCC (waiting for result)

  9. CG15252 enhancer: 100bp with 2 TCGTG sites DT S16 DT S17

  10. CG13196 500bp 350bp 100bp? 490bp CG13196-500 CG13196-350

  11. Ungoing..... CG15252 DT expression depends on Sal? • CG15252-GFP expression in Sal mutant? • Compare CG15252 expression in btl-gal4; UAS-dys and btl-gal4, UAS-sal; UAS-dys • Compare CG15252 expression in btl-gal4; UAS-dys and btl-gal4, UAS-kni; UAS-dys Co-activator or repressor of Dys::Tgo? • Compare enhancer of CG13196 and CG15252, identify potential binding sites (drifter?)

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