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Hemoglobin determination Biotest Hemoglobin System Accuracy and precision

Hemoglobin determination Biotest Hemoglobin System Accuracy and precision. Biotest Medizintechnik GmbH. Products. Cuvette Biotest Hemoglobin Measuring device Biotest Hemoglobin Tester. Cuvette Blood capillary, reagent and measuring cuvette in one. Biotest Hemoglobin Tester

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Hemoglobin determination Biotest Hemoglobin System Accuracy and precision

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  1. Hemoglobin determination Biotest Hemoglobin SystemAccuracy and precision Biotest Medizintechnik GmbH

  2. Products • Cuvette • Biotest Hemoglobin • Measuring device • Biotest Hemoglobin Tester

  3. Cuvette Blood capillary, reagent and measuring cuvette in one. Biotest Hemoglobin Tester Photometer with integrated control and digital display Function

  4. Function - Cuvette • Chemical reaction Haemolysis by sodium desoxycholat Sodium nitrite Sodium azid Methemo-globin Free Haemoglobin Azid- Methaemo-globin Erythrocytes

  5. Function - Cuvette • Absorbance curve

  6. Measurements

  7. 20,00 y = 1,0064x - 0,0773 R² = 0,9992 15,00 10,00 5,00 5,0 g/dl 10,0 g/dl 15,0 g/dl 20,0 g/dl Measurements Linearity and precision, n = 20 measurements by each blood sample. Biotest Hemoglobin System, g/dl Mean value HiCN, i g/dl

  8. Measurements

  9. Evaluation Function - Tester • Photometer; two wave lengths • Evaluation and calculation • Reading

  10. Evaluation Self calibration • 100% green light, 100% red light and dark current • Compared to moment of calibration • If necessary automatic adjustment of LED current

  11. Resolution Max deviation = +/- 0,75 % or +/- 30 steps Absorption 256 g/L 0 = 100 % light 470 – 880 nm 12 bits = 4000 ”steps” Resolution

  12. Calibration Absorption 256 g/L Two points of calibration for linearity; 80 and 220 g/L Three control levels for linearity 120, 150, 180 g/L 0 = 100 % light 470 – 880 nm 12 bits = 4000 ”steps”

  13. Function - Tester • Calculation of Hb Extinction Turbidity Turbidity 500 570 600 700 800 880 Wave length [nm]

  14. Capillary blood Hb Time 0 min 1 min 2 min 3 min

  15. Results - Introduction • Accuracy • How close to true value is the result • Precision • How close to each other are repeated measurements with the same sample

  16. Results - Introduction • Accuracy and precision High accuracy Low precision Low accuracy High precision High accuracy High precision

  17. Results - Introduction • Accuracy: • Deviation (mean) in g/L • Preferred; deviation in % • Precision: • Standard deviation SD • Preferred; Coefficient of variation, CV %

  18. Results Precision with control blood (n=50) 30 Mean value 12,39 g/dl SD 0,084 g/dl CV 0,68 % 25 20 Numbers 15 10 5 0 12,1 12,2 12,3 12,4 12,5 12,6 12,7 Result [g/dl]

  19. Features • Cuvette • Aimed for Hb-measurement with Biotest-Hemoglobin-Tester and compatible instruments • High accuracy and precision • Can be used for venous, capillary and arterial blood • Developed to be price worth due to latest production and control technique

  20. Features • Tester • Automatic calibration: no calibration or test cuvette needed • Cuvette holder minimizing the risk of contamination • Automatic monitoring of power supply with fast loading of batteries (1 hour loading > 40 hours of measuring) • Automatic shut off with latest value saved • Measures only cuvettes with properly haemolysed blood

  21. Summary • High accuracy and precision • Easy and reliable system • Long time use on battery power

  22. Results - Introduction

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