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計畫主持人:江善宗 共同主持人:許 濤 PowerPoint PPT Presentation


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教育部顧問室 『 生物技術科技 』 教育改進計畫 夥伴學校計畫成果報告. 重點規劃領域:  植物生物技術. 計畫主持人:江善宗 共同主持人:許 濤. 申請機關:國立臺灣海洋大學 參與科系所: 海洋大學: 生物科技研究所 水產養殖學系 海洋生物研究所 中央研究院: 植物研究所 台灣大學: 海洋研究所. 重點領域及特色. 本校在生物技術教育所建立之教學環境特色. 強化基礎科學教育,為培養生技產業人才奠定良好根基:.

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計畫主持人:江善宗 共同主持人:許 濤

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  • Native PAGESDS-PAGE



  • (MALDI-TOF)

  • MALDI-TOF

  • (MS/MS)

  • MALDI-TOFMS/MS


  • DNA

  • DNA

  • DNA

  • DNA

  • DNA

  • DNADNA

  • DNA

DNA


  • Cleaning of glassware by 50% nitric acid

  • Preparation of 12.5% SDS-polyacrylamide gel

  • Preparation of green algae crude extract proteins

  • Handling of pH3-10 IPG strip

  • Rehydration of IPG strip at low voltage

  • Separation of algal proteins by iso- electrofocusing

  • Separation of algal proteins by SDS-PAGE

  • Identification of proteins by silver staining



  • DNA


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  • cDNA

  • Polymerase Chain Reaction (PCR)

  • SOD()(PCR)

  • DNADNA

  • PCR

  • PCRpGEM-T

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  • (transformation)

  • DNA

  • DNA

  • GCG

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UV induced protein

2D Electrophoresis of Algal Proteins

2D Electrophoresis of Algal Proteins


Designed HG-GCT-AAS system


GCT compartment


Chromatogram with optimized parameters


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