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計畫主持人:江善宗 共同主持人:許 濤 PowerPoint PPT Presentation


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教育部顧問室 『 生物技術科技 』 教育改進計畫 夥伴學校計畫成果報告. 重點規劃領域:  植物生物技術. 計畫主持人:江善宗 共同主持人:許 濤. 申請機關:國立臺灣海洋大學 參與科系所: 海洋大學: 生物科技研究所 水產養殖學系 海洋生物研究所 中央研究院: 植物研究所 台灣大學: 海洋研究所. 重點領域及特色. 本校在生物技術教育所建立之教學環境特色. 強化基礎科學教育,為培養生技產業人才奠定良好根基:.

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計畫主持人:江善宗 共同主持人:許 濤

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2.

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(1)


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(2)

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rotor


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91


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  • Native PAGESDS-PAGE


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  • (MALDI-TOF)

  • MALDI-TOF

  • (MS/MS)

  • MALDI-TOFMS/MS


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  • DNA

  • DNA

  • DNA

  • DNA

  • DNA

  • DNADNA

  • DNA

DNA


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  • Cleaning of glassware by 50% nitric acid

  • Preparation of 12.5% SDS-polyacrylamide gel

  • Preparation of green algae crude extract proteins

  • Handling of pH3-10 IPG strip

  • Rehydration of IPG strip at low voltage

  • Separation of algal proteins by iso- electrofocusing

  • Separation of algal proteins by SDS-PAGE

  • Identification of proteins by silver staining


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  • DNA


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()()

  • cDNA

  • Polymerase Chain Reaction (PCR)

  • SOD()(PCR)

  • DNADNA

  • PCR

  • PCRpGEM-T

()

  • (transformation)

  • DNA

  • DNA

  • GCG

()


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UV induced protein

2D Electrophoresis of Algal Proteins

2D Electrophoresis of Algal Proteins


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Designed HG-GCT-AAS system


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GCT compartment


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Chromatogram with optimized parameters


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A

B

B

A


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91

(1)

(2)


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(1)

(2)

(3)


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(1)

(2)(protoplast)

(3)cDNAcDNA


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92

  • 92

  • 92(1)(2)(3)30%70%919278


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92

(1)

(2)9192


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(4)


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http://ind.ntou.edu.tw/~cfs/BiotechCenter/Index.htm


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  • 30%70%50%


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(3)

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3

II.

3

III.

3


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I.

(1)

II.

(1)

III.

(1)

IIII17


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