The Biological Role of Abscisic Acid in Precocious Seed Germination. Present by Collin Mecke April 1 st 61.325. Introduction to ABA. -Synthesis of ABA occurs in the chloroplasts and other plastids using the terpenoid pathway (fig 23.2) Detected in all parts of the plant
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-Synthesis of ABA occurs in the chloroplasts and other plastids using the terpenoid pathway (fig 23.2)
Bioassay-Measuring coleoptile growth inhibition. Minimum detection is 10-7 M
-Closure of stomata (10-9M)
Physical Methods- Gas Chromatography (10-13g)
-High Performance Liquid Chromatography
Immunoassay-Recognition of ABA by antibodies
-Immature embryos were removed from seeds and cultured midway through development (before the onset of dormancy)
-These embryos germinated precociously (without passing through normal dormancy)
- When ABA was added to the medium precocious germination did not occur.
1. Transcriptional activator of ABA-inducible gene
2. ABA-independent repressor of germination specific