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Measuring small and very small volumes

Measuring small and very small volumes. Lab Manual 3A and 3B. timeline. Monday—Lecture, activity Tuesday—Lab 3A Thursday—3A individual skills quiz Monday—3A due, Lab 3B Tuesday—3B individual skills quiz Thursday—3B due.

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Measuring small and very small volumes

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  1. Measuring small and very small volumes Lab Manual 3A and 3B

  2. timeline Monday—Lecture, activity Tuesday—Lab 3A Thursday—3A individual skills quiz Monday—3A due, Lab 3B Tuesday—3B individual skills quiz Thursday—3B due

  3. Write-up (same for 3a and 3b, but turn in each with a separate cover sheet) • Procedures • Reflection/Analysis—1 paragraph • Thinking Like a Biotechnician (#3 done as quiz for 3A & 3B, skip #2 for 3b)

  4. Tutorials • Using a pipet demo (tutorial 2) • Micropipet (tutorial 3)

  5. Lab 3a tips & changes • Label tubes A-D • Solution I (red) II (yellow) III (green) IV (blue) V (clear) • Skip Procedure 6 • Work in pairs • Meniscus—measure from the bottom of the meniscus • Add smallest volume first • Label the tops of the tubes (sample, initials, date) • Use the smallest pipet possible • Rinse pipet between colors, wrist flick remaining drops • At the end of class, rinse out test tubes and dry them on your rack

  6. Tips for 3b • Work as a group of 2, take turns • Solution I (red) II (orange) III (green) IV (blue) V (clear) • Add smallest volume first • 10—biggest # is tens place, 100-biggest # is hundreds place, 1000—biggest # is thousands place (but this depends on the style of pipet) • Change tips—put in beaker and then in trash • Have recipient tube ready • Label the tops of the tubes (sample, initials, date)

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