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黄曲霉毒素的测定 PowerPoint PPT Presentation


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黄曲霉毒素的测定. 黄曲霉毒素( Aflatoxins, 简写 AFT ),是黄曲霉、寄生曲霉及温特曲霉等产毒株的代谢产物,是一群结构类似的化合物。目前已发现 17 种黄曲霉毒素,根据其在波长为 365nm 紫外光下呈现不同颜色的荧光而分为 B 、 G 二大类,其中 B 大类在氧化铝薄层板上于紫外光下呈现蓝色荧光,而 G 大类则呈绿色荧光。. 黄曲霉毒素属剧毒物质,其毒性比氰化钾还高,也是目前最强的化学致癌物质。其中 AFT B1 的毒性和致癌性最强,帮其在食品中允许量各国都有严格规定。 AFT 主要污染粮油及其制品,如花生、花生油、玉米、大米、棉籽等被污染严重。.

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黄曲霉毒素的测定

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  • Aflatoxins,AFT17365nmBGBG


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  • AFT B1

  • AFT


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  • ppb10-6

  • 20

  • 20

  • 10

  • 5


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1990AOAC(association of official agricultural chemists)


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B1365nmB1

Bt0000 4ug5ugKg


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5cm20cm

(25cm6cm4cm)

100125w365nm(2)


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  • (3060)(6090)


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G5-20%

H

HF254 254

GF254


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B1

  • 1B1112mg AFTB12mL100mL410ugmL100ugmL350nmAFTB(98+2)19 800 .


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2B

I(1.0ugmL)1mL AFTB(10.0ugmL)10mL

(0.2ugmL)I1mL5mL

(0.04ugmL)1mI5mI

B


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  • 100g500mL80g(Na2CO3H2O)500mL25gL

  • 1

  • 2


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(1)

05lkg2010


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(2)

20.00g250mL30mL100mL

30min


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20.00mL125mL20mL2min10g50mL5mL


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6523min1mL2mL


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  • 13gG2312min5cm20cm0.25mm315min1002h23d


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3cm4lcm3mm

10L AFTB(004gmL)

20L20L+10L 0.04gmL AFTBl20L+10L 0.02tLgm L AFTB


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  • 10mL12cm10mL(8+92)1012cm

    aAFTB1AFTB1AFTB1AFTB10000 4gAFTB1AFTB10002ug

    bAFTB1AFTBl<5ugkg


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B1AFTB10.1

10uL 004ugmL AFTBl 20L5min2min(40)

10uL 0.04ugmL AFTBl20L


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AFTB1

AFTB1AFTB1(0.00 04g)AFTB15~gkg

l0uL AFTB1(0.04ugmL)

10uL

15uL

20uL


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4)

X =

X AFTBlugkg

V1mL

V2mL

D

m1g

0.0004AFTBlug


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AFTB1AFTB1(8+92)


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(1)3cm081cm10uL AFTB1(0.04ugmL)2.83cm20uLl0uL AFTBl(0.04ugmL)10uL AFTB1(0.02ugmL)


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(2)

a10mLl2

b(892)1012cm


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aAFTB1AFTBl<5ugkg.

bAFTB1


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0.81cm10u LATTB1(0.04ugmL)2.83cm20uL20gL10uLAFTBl(0.04ugmL)5min2min(40)AFTB1AFTB1(4).


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(5)

AFTBl(5)AFTB1

3.2.3

3.2.4

(25gL)


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