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セボフルレンスプレー麻酔を利用したラットの簡易保定

セボフルレンスプレー麻酔を利用したラットの簡易保定. 戸部善継、石郷岡清基、松田幸久 1) 、輪湖雅彦 2) 1)秋田大学バイオサイエンス教育・研究センター動物実験部門 2)秋田大学医学部感覚器学講座皮膚科学・形成外科学分野. Convenient sevoflurane spray anesthesia for restraint of rat. Y. Tobe, S. Ishigouka, Y. Matsuda 1) , M. Wako 2) 1) Bioscience Education and Research Center,

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セボフルレンスプレー麻酔を利用したラットの簡易保定

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  1. セボフルレンスプレー麻酔を利用したラットの簡易保定セボフルレンスプレー麻酔を利用したラットの簡易保定 戸部善継、石郷岡清基、松田幸久1)、輪湖雅彦2) 1)秋田大学バイオサイエンス教育・研究センター動物実験部門 2)秋田大学医学部感覚器学講座皮膚科学・形成外科学分野

  2. Convenient sevoflurane spray anesthesia for restraint of rat Y. Tobe, S. Ishigouka, Y. Matsuda 1), M. Wako 2) 1)Bioscience Education and Research Center, Division of Animal Experiments, Akita University 2)Department of Dermatology and Plastic Surgery. Division of Sensory Organ. Akita University School of Medicine

  3. Materials Container Spray bottle Sevoflurane

  4. Materials SD rats ♂ ♀ 8〜13 weeks

  5. Materials Fukuda Denshi Co Malti-gas Monitor

  6. Methods

  7. Concentration of O2 &CO2 Sec. 30 60 90 120 150 180 ♂ 20.0 20.0 19.8 19.3 19.2 19.0 O2 (%) ♀ 20.0 20.0 19.8 19.7 19.7 19.6 ♂ 0.31 0.55 0.63 0.82 1.0 1.2 CO2 (%) ♀ 0.28 0.35 0.37 0.54 0.62 0.67

  8. Discuss

  9. Conclusion Refinementin restraint of rats Safetyfor rats and researchers Low costin experiments

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