b lymphocyte mediate recognition of antigens and the immune response
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B lymphocyte-mediate recognition of antigens and the immune response. Contents. B cell-mediate immune response to T-dependent antigen B cell-mediate immune response to T-independent antigen The general rule of humoral immune response. Humoral immunity:

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Presentation Transcript
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Contents

  • B cell-mediate immune response to T-dependent antigen
  • B cell-mediate immune response to T-independent antigen
  • The general rule of humoral immune response
slide3

Humoral immunity:

A immune response mediated by B lymphocyte-secreted antibodies. Its funtion is to neutralize and eliminate the antigens which induced the antibodies.

slide4

B cell-mediate recognition to antigens

B cell-mediate recognition to TI antigens

TI-antigen: T-independent antigen

This type of antigen can activate B cells without the T cell help,

and most of them are bacterial polysaccharides and lipopolysaccharide.

1. TI-1 aitigen

2. TI-2 antigen

slide5

TI-1 antigen: also named B cell mitogen

  • Contains B cell mitogen and repeated B epitopes
  • Can induce immature and mature B cell-mediate immune response
  • In high concentrations, can induce polyclonal B-cell activation
  • In lower concentrations, only the BCR-binding TI-1 antigens can activate B cells
  • Not able to induce Ig class switch, antibody affinity maturation and memory B cells
slide6

TI-1 antigen in high concentrations

B-cell mediate recognition and immune response to TI-1 antigens

polyclonal B-cell activation

non-specific antibody response

TI-1 antigen in lower concentrations

Specific antibody response to TI-1 antigen

slide7

TI-2 antigen

  • Only contains multitude repeated B-cell epitopes
  • Merely induces mature B cell-mediated immune response
  • Unresponses or hyporesponsive to infantile B cells
  • Activates the B-1 cells
slide8

TI-2 antigen alone activates B cells to secrete antibodies

cell

B cell-mediate recognition and immune response to TI-2 antigens

Plasma cell

slide9
TI antigen mainly activates CD45+ B1 cells
  • The produced antibodies are mainly IgM
  • No MHC-restriction, without APC and Th cell help
  • Can not induce antibody class switch, affinity maturation and memory B cells
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(二)B cell-mediate recognition to TD antigens

  • BCR binds to antigen specifically and transfer the antigen stimulatory signal into B cells
  • BCR specific binding to the antigen and intake it through internalization, then the antigen was degradated into peptides and the peptide-MHC-II molecule complex was formed, which can be recognized by antigen specific Th cells
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二. Activation of B Cells

1)The specific antigenic recognition signal of B cell activation (first signal)

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B cell coreceptor complex

The first signal of B cell activation

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2)Costimulatory signal of B cell activation (second signal)

With the help of T cells

1.The activation of naïve Th cell

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2. The combination of Th cell and specific B cell

TCR presented on Th cells specifically recognizes and binds antigenic peptide-MHC-II complex displayed on B cell surface

The interaction of many adhesion molecular pairs makes the binding tighter.

slide16

☆Direct contact

T cells express CD40L,LFA-1,CD28, et al.

CD40/CD40L,ICAM-1/LFA-1,B7/CD28

The most important molecule is CD40L(CD154)

slide17

B cell

Th cell

antigen

Signal 1

Th activation

Presentation of antigen

Signal 2

Signal 2

Signal 1

B activation

slide18

3. The activation of specific B cells

Th cells can be induced to express many membranous molecules, for instance CD40L

The dark zone formation of the germinal center in lymph follicles

The clonal proliferation of B cells

B cells are differentiated to the cells in the germinal center

The formation of memory B cells

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The function of cytokines

IL-2, IFN- secreted by Th1 cells

IL-4、IL-5、IL-6 secreted by Th2 cells

slide20

TD antigen

B cell

Th cell

cytokines

B cell

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三. The signal transduction of B cell activation

(一)the antigen recognition structure of B cells

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4. differentiation and maturation of B cells in the germinal center

  • FDC, B cell interaction ---B cell uptake antigens.
  • Antigen uptaken, process and presentation by B cells.
  • T, B cell interaction---Th cell activation.
  • CD40L upregulation and cytokine secretion in activated Th cells---further differentiation and maturation of B cells.
slide24

Centroblasts

centrocytes

Germinal center

Blast proliferated B cells:

mantle zone formation

Non-proliferated B cells:

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(1) receptor editing

Further immunoglobulin gene rearrangement in seconary lymph organs---some potentially self-reactive B cells may be rescued.

Otherwise, self-reactive B cells undergo apoptosis or anergy.

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(2) somatic hypermutation and

affinity maturation

Ig+ B cells proliferate rapidly, and their already rearranged receptor genes undergo further diversification.

Some further diversifided Ig+ B cells acquir more affinitive BCR.

slide28

B cell maturation, migration and GC formation

B cell proliferation

somatic hypermaturation

antigen recognition and affinity maturation

no antigen loading B cells undergoing cell death

generation of plasma or memory B cells

B cell differentiation and maturation in germinal center

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(4) destiny of maturated GC B cells

  • plasma cell, PC
  • memory B cell
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(6) Humoral immune response

  • Primary immune response
  • Secondly immune response or
  • anamnestic response
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Primary immune response

☆lag phase

☆log phase

☆steady-state phase

☆decline phase

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Antigen

Steady-state phase

Lag phase

Antibody titer

Log phase

Decline pahse

Time

slide34

Secondly immune response or anamnestic response:

  • Short lag phase
  • longer steady-state phase
  • lower dose of antigen needed
  • mainly IgG
  • highly avidity
slide36

Master the general rule of B cell mediated immune response angainst TD-Ag.

  • Master similarities and differences betweenTI-Ag and TD-Ag inducing B cell immune response.
  • Understand mucosal immune response.
  • Understand mature , somatic hypermutation and affinity maturation and class switch of B cell.
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