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Macrophage Polarization

Macrophages reveal specific phenotypes and functions in response to different triggers. In the macrophage polarization assay, specific stimuli co-cultured with isolated bone marrow-derived or peritoneal macrophages are provided to generate different polarized macrophage subsets. <br>https://www.creative-biolabs.com/car-t/macrophage-polarization-assay.htm

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Macrophage Polarization

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  1. Macrophage Polarization • https://www.creative-biolabs.com/car-t/

  2. Background Macrophages belong to a heterogeneous population of innate myeloid cells which is involved in disease and health. They are the most functional diverse cells of the hematopoietic system. Macrophages exert multiple functions in human, including responding to pathogens and modulating the adaptive immune response, induction and resolution of inflammation, tissue repair, and homeostasis. Macrophages own appealing plasticity characteristic. Therefore, in response to different stimuli, different populations of macrophages with various physiological and pathological roles can be developed. Creative Biolabs offers the macrophage polarization assay to induce different macrophage subsets.

  3. Assay of Macrophage Polarization Macrophages reveal specific phenotypes and functions in response to different triggers. In the macrophage polarization assay, we provide specific stimuli co-cultured with isolated bone marrow-derived or peritoneal macrophages to generate different polarized macrophage subsets. Depending on the types of stimuli that macrophages are exposed to, they are able to polarize to M1 (pro-inflammatory) or M2 (anti-inflammatory) macrophages (see Fig 1). Based on the stimuli and the achieved transcriptional changes, the M2 macrophages can be classified into four subdivisions - M2a, M2b, M2c, and M2d. In this assay, except for different macrophage polarization by adding certain stimuli, we also provide support for our clients with verification of each polarized macrophage-based on the distinctly expressed surface markers and/or produced cytokines.

  4. Here we list the different stimuli used to trigger different macrophage populations. ➢ M1 macrophage: IFNγ and LPS➢ M2a macrophage: IL-4 or IL-13➢ M2b macrophage: Immune complexes and LPS➢ M2c macrophage: Glucocorticoids or IL-10➢ M2d macrophage: Adenosines or IL-6

  5. Figure 1 Macrophage polarization. (Zanluqui, et.al. 2015)

  6. Highlight Features ➢ Senior Ph.D. scientists and experienced team➢ Skillful technologies and advanced platforms➢ High quality but competitive price➢ Timely after-sales service ➢ Customized assay design

  7. About Creative Biolabs • With the exponentially increased demand in modern novel medicine, the CAR and TCR immune cell therapy has been one of the most promising approaches for target-specific cancer treatment. With integrated global resources in all aspects, Creative Biolabs provides one-stop full CAR T/NK Cell therapy development service, including early non-clinical research, process development, pre-clinical animal model testing, cGMP manufacturing, and phase I-III clinical trials.

  8. Contact • 45-1 Ramsey Road, Shirley, NY 11967, USA • Email: marketing@creative-biolabs.com • www.creative-biolabs.com/car-t

  9. ThankYou

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