Isolation of Rare Circulating Tumor Cells in Cancer Patients by Microchip Technology
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Isolation of Rare Circulating Tumor Cells in Cancer Patients by Microchip Technology Toner, et al. 2007. Stephanie Bachar Matt Luchette. Background: Circulating Tumor Cells. CTCs: tumor cells shed into the blood stream Motivation for detection Study biology of metastasis

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Isolation of Rare Circulating Tumor Cells in Cancer Patients by Microchip TechnologyToner, et al. 2007

Stephanie Bachar

Matt Luchette


Background circulating tumor cells l.jpg
Background: Circulating Tumor Cells by Microchip Technology

  • CTCs: tumor cells shed into the blood stream

  • Motivation for detection

    • Study biology of metastasis

    • Diagnose, detect, monitor cancer in patients without invasive biopsy

The bone marrow niche: habitat to hematopoietic and mesenchymal stem cells, and unwitting host to molecular parasites

Y Shiozawa, A M Havens, K J Pienta and R S Taichman


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Motivation for the “CTC-Chip” by Microchip Technology

  • Shortcomings prior to “CTC-Chip”

    • Accuracy: 20-60%

    • Purity: 0.1%

    • Non-viable cells

Photoacoustic detection of CTCs. Viator lab at University of Missouri

http://web.missouri.edu/~viatorj/tumor.htm


How to use the ctc chip l.jpg
How to use the “CTC Chip" by Microchip Technology

  • Flow sample through CTC Chip

  • EpCAM coated microposts

  • Verify with markers

Pressure Control

Blood on Rocker

CTC Chip

Toner 2007

EpCAM

Non-CTCs

CTCs

Micropost

Cytokeratin

CD45


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Instrument Design: by Microchip Technologyin silico

  • Maximize CTCs detected

    • Flow Velocity

    • Shear Force

  • Vary micropost characteristics

  • Theoretical capture efficiency of 65%

Toner 2007


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Laboratory Results by Microchip Technology

  • 1 target cell/10^9 blood cells

  • Purity of capture ~60% (competitors = 0.1%)

  •  Efficiency of capture ~65% (competitors = 35%)

  • Captured cells were viable and could be further manipulated

Capture purity per type of cancer

Toner 2007


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Clinical Results by Microchip Technology

  • Identified CTCs in 99% cancer pts (competitors =  20-60%)

  • no false positives

  • Change in number of CTCs correlated with clinical path

  • No pre-processing required

Change in CTCs captured per mL (red) with lung cancer patient mirrors change in tumor size (blue)

Toner 2007


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Concerns and next steps by Microchip Technology

  • How cells used afterwards?

  • How to mass produce chips?

  • Financially viable?

  • How reliable is EpCAM as a caner cell marker?

  • FDA approval

Paper tree-branch glucose and protein assay for urine

technologyreview.com


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Recap/Applications by Microchip Technology

  • New diagnostic and prognostic tool for cancer patients

  • Provide alternative to biopsies for areas with low resources

  • Use to isolate and further study CTCs

MRI of 67-year old patient with pancreatic cancer with the pancreatic tumor indicated

imagingeconomics.com


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Works Cited by Microchip Technology

--imagingeconomics.com

--technologyreview.com

--http://web.missouri.edu/~viatorj/tumor.htm

--The bone marrow niche: habitat to hematopoietic and mesenchymal stem cells, and unwitting host to molecular parasites Y Shiozawa, A M Havens, K J Pienta and R S Taichman


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