Instrumentation and control
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Instrumentation and control. Parameters: to be monitored and controlled Temperature Pressure Agitator shaft power Flowrate Liquid level Viscosity Turbidity pH Redox potential Ion concentration DO Read pages 308-310 in the text book. Summary of Bioreactor Design and Operation.

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Instrumentation and control

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Instrumentation and control

Instrumentation and control

  • Parameters: to be monitored and controlled

  • Temperature

  • Pressure

  • Agitator shaft power

  • Flowrate

  • Liquid level

  • Viscosity

  • Turbidity

  • pH

  • Redox potential

  • Ion concentration

  • DO

    Read pages 308-310 in the text book.


Summary of bioreactor design and operation

Summary of Bioreactor Design and Operation

  • Modified batch and continuous reactors

    - Chemostat with cell recycle

    Keep high cell mass concentration in the reactor.

    Production of biomass and low-value product

    - Fed-batch

    Maintain low substrate concentration

    Secondary metabolites, prevent catabolite repression

    - Multi-stage chemostat reactor

    Separate cell growth and product formation

    Secondary metabolites, genetically modified cell culture


Instrumentation and control

Summary of Bioreactor Design and Operation

  • Modified batch and continuous reactors

    - Chemostat with cell recycle

(qp=0, kd ≈0, X0=0,Monod equation is applied):


Instrumentation and control

Summary of Bioreactor Design and Operation

  • Modified batch and continuous reactors

    - Fed-batch

(qp=0, kd ≈0, Monod equation is applied):


Instrumentation and control

Summary of Bioreactor Design and Operation

  • Modified batch and continuous reactors

    - Multi-stage chemostat reactor


Instrumentation and control

Summary of Bioreactor Design and Operation

  • Immobilized cell system

    Advantages and disadvantages

  • Operation consideration

    agitation and aeration, determination of volumetric mass transfer coefficient kLa,

    heat removal, foam, etc.


Instrumentation and control

Summary of Bioreactor Design and Operation

  • Scale up/down: geometric and dynamic similarity:

    In scale-up/down of a stirred-tank reactor, the design calculations are as follows:

  • Determine the scale-up/down factor Dp/Dm

  • Calculate the dimensions of the prototype (height H and diameter Dt of tanks, impeller diameter Di) by multiplying that of the model with the scale-up/down factor.

    -Select criterion related to dynamic properties and keep it constant in both the model and the prototype.

    -Determine the parameters such as impeller speed for the scale-up/down reactor.


Instrumentation and control

Summary of Bioreactor Design and Operation

  • Sterilization

    liquid: thermal inactivating

1- P0(t)= 1-[1-e-kdt]N0

kd = αe-E0d/RT

From the above equation:

  • Known N0, T, t, determine Kd, the probability of an unsuccessful sterilization is determined.

  • Given N0, T, acceptable probability of failure e.g. 10-3, required time can be determined

  • Higher Kd tends to achieve low probability of sterilization failure. Normally at 121oC.

    Kd of vegetative cells > 1010 min-1, spores 0.5-5 min-1. The major concern is spores.


Instrumentation and control

Summary of Bioreactor Design and Operation

  • Sterilization

    Degradation of important compounds in the medium by thermal inactivating

ln C/C0=-kdt

where C and C0 are concentrations of the component at time t and t=0, respectively.

kd is the degradation rate constant.

kd = αe-E0d/RT

To determine the components remaining active:

the temperature T → determine kd → with known t, determine C.


Instrumentation and control

Summary of Bioreactor Design and Operation

  • Bioreactor Instrumentation and control


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