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Biotechnology/Cloning. Reproductive cloning. Reproductive cloning. Therapeutic cloning. Therapeutic cloning. http://www.rndsystems.com/dam_public/6515.jpg. Recombinant DNA technology. DNA isolated from two sources and cut by the same enzyme. Plasmid (vector). Gene of interest.

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Biotechnology/Cloning

Reproductive cloning




Therapeutic cloning

http://www.rndsystems.com/dam_public/6515.jpg


Recombinant DNA technology

DNA isolated from

two sources and cut by

the same enzyme

Plasmid

(vector)

Gene of interest

Some uses of genes:

Some uses of proteins:

Recombinant

DNA

Gene for pest

resistance

Protein for

dissolving

clots

Gene for

toxic-cleanup

bacteria

Protein for

“stone-washing”

jeans

Transgenic organisms

Useful products


RestrictionEnzymes


First Cloning Experiment

Cohen et al., 1973

“Recombinant DNA”


Cloning into pBR322

Cloning Vectors: pBR322 plasmid



Palmiter et al. (1982) Dramatic growth of mice that develop from eggs microinjected with metallothionine-growth hormone fusion genes. Nature 300: 611-615

“Supermice”



First Glowing Pet

Weeding Out Land Mines


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