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LS1B

LS1B. Section One. Schedule. Introductions/Attendance Pre-lab Quiz Section Overview/Policies Yeast Complementation Review Yeast Complementation Lab Work Review Practice Problems Mitosis Review Mitosis Lab Work Clean up. Yeast Complementation. What is Yeast?. Yeast Complementation.

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LS1B

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  1. LS1B Section One

  2. Schedule • Introductions/Attendance • Pre-lab Quiz • Section Overview/Policies • Yeast Complementation Review • Yeast Complementation Lab Work • Review Practice Problems • Mitosis Review • Mitosis Lab Work • Clean up

  3. Yeast Complementation What is Yeast?

  4. Yeast Complementation

  5. Yeast Complementation Why? What are we trying to find out? • For six mutant strains,which step (enzyme) in the histidine pathway is disrupted? • Same step or different steps between the different strains?

  6. Yeast Complementation What do we already know? • None of them can grow without histidine (ie they can’t make it themselves) • They are of mating type α

  7. Yeast Complementation What do we have? • Six other strains in which the histidine pathway is also disrupted but we know which step (enzyme)! • They are of mating type a

  8. Yeast Complementation • What to do? • Mate the ones with unknown mutations (α) with those with known mutations (a). Growth without Histidine • If we see growth: different genes (enzymes, steps in the histidine pathway) • If we don’t see growth: same gene (enzyme, step in the histidine pathway)

  9. Yeast Complementation • What we are going to do today • Streak out known and unknown HIS- mutants Why? Allow us to mate them in grid pattern next week

  10. Schedule • Introductions/Attendance • Pre-lab Quiz • Section Overview/Policies • Yeast Complementation Review • Yeast Complementation Lab Work • Review Practice Problems • Mitosis Review • Mitosis Lab Work • Clean up

  11. Mitosis Interphase Uncondensed chromosomes replicate

  12. Mitosis Prophase Chromosomes condense and move towards metaphase plate

  13. Mitosis Metaphase Chromosomes line up on metaphase plate

  14. Mitosis Anaphase Sister chromatids separate

  15. Mitosis Telophase Chromosomes finish moving to opposite sites

  16. Mitosis Cytokinesis then Interphase

  17. Mitosis

  18. Mitosis

  19. Schedule • Introductions/Attendance • Pre-lab Quiz • Section Overview/Policies • Yeast Complementation Review • Yeast Complementation Lab Work • Review Practice Problems • Mitosis Review • Mitosis Lab Work • Clean up

  20. Clean Up • Gas off, Bunsen burner disconnected • All streaked plates labeled, taped, in drawer • All slides in glass disposal • Remaining tooth picks up at front on red tray • Microscopes turned off, unplugged • Cameras labeled with names and put on front desk • Stain, unused slides, unused coverslips, remaining onion, tweezers, scissors, probe, etc at front of room on or near red tray • Carnoy and HCL left on desk top • Desktops wiped down, chairs pushed in THANK YOU!!

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