John A.G. Briggs
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John A.G. Briggs Retrovirus structure and assembly. The structural genomics of HIV. Gag. Immature virion. Immature HIV. p17. p24. p7. p2. p1. Gag cleavage – can be inhibited. maturation. MMTV. Immature virion. HIV. 2 pathways of Retrovirus formation. Immature virion.

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John A.G. Briggs Retrovirus structure and assembly

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John a g briggs retrovirus structure and assembly

John A.G. Briggs

Retrovirus structure and assembly


The structural genomics of hiv

The structural genomics of HIV


John a g briggs retrovirus structure and assembly

Gag


John a g briggs retrovirus structure and assembly

Immature virion


John a g briggs retrovirus structure and assembly

Immature HIV


John a g briggs retrovirus structure and assembly

p17

p24

p7

p2

p1

Gag cleavage – can be inhibited


John a g briggs retrovirus structure and assembly

maturation


John a g briggs retrovirus structure and assembly

MMTV

Immature virion

HIV

2 pathways of Retrovirus formation.


John a g briggs retrovirus structure and assembly

Immature virion

Internal scaffold domain

Cytoplasmic retention motif


John a g briggs retrovirus structure and assembly

MMTV

Immature virion

HIV

Envelopment?


John a g briggs retrovirus structure and assembly

cytosol


John a g briggs retrovirus structure and assembly

cytosol


John a g briggs retrovirus structure and assembly

maturation


John a g briggs retrovirus structure and assembly

Finch/Sundquist lab


John a g briggs retrovirus structure and assembly

Core formed from ~1200 capsid proteins

Arranged in ~96Å hexagonal lattice


John a g briggs retrovirus structure and assembly

What is the structure of the lattice here?

maturation


John a g briggs retrovirus structure and assembly

Immature HIV-1

In-vitro assembled


John a g briggs retrovirus structure and assembly

If it is made from 80A hexagons, with six proteins per hexagon then 3400 ±200 gag proteins


John a g briggs retrovirus structure and assembly

Scanning Transmission Electron Microscopy

Mean mass: 165 ±29MDa

3800 ± 700 gag proteins


John a g briggs retrovirus structure and assembly

145nm immature virion formed from ~5000 Gag proteins

Arranged in ~80 Å hexagonal lattice


John a g briggs retrovirus structure and assembly

Immature virion

Tighter hexagonal lattice with 8 nm unit cell.

~5000 gag proteins form a complete shell for a 145 nm virus

Mature virion

Looser hexagonal lattice with 9.6 nm unit cell.

~1200 CA proteins form the conical core, leaving a large number elsewhere


John a g briggs retrovirus structure and assembly

Virology

University of Heidelberg

University of Oxford

Cornell University

Hans-Georg Kraüsslich

Barbara Müller

Marko Lampe

Bärbel Glass

Marc Johnson

Volker Vogt

Quentin Sattentau

Electron Microscopy

University of Oxford

Max-Planck Institute for Biochemistry

Stephen Fuller

Kay Grünewald

Friedrich Förster

Thomas Wilk

Giulia Zanetti


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