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John A.G. Briggs Retrovirus structure and assembly

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John A.G. Briggs Retrovirus structure and assembly. The structural genomics of HIV. Gag. Immature virion. Immature HIV. p17. p24. p7. p2. p1. Gag cleavage – can be inhibited. maturation. MMTV. Immature virion. HIV. 2 pathways of Retrovirus formation. Immature virion.

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John A.G. Briggs

Retrovirus structure and assembly

slide6

p17

p24

p7

p2

p1

Gag cleavage – can be inhibited

slide9

MMTV

Immature virion

HIV

2 pathways of Retrovirus formation.

slide11

Immature virion

Internal scaffold domain

Cytoplasmic retention motif

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MMTV

Immature virion

HIV

Envelopment?

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Core formed from ~1200 capsid proteins

Arranged in ~96Å hexagonal lattice

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Immature HIV-1

In-vitro assembled

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If it is made from 80A hexagons, with six proteins per hexagon then 3400 ±200 gag proteins

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Scanning Transmission Electron Microscopy

Mean mass: 165 ±29MDa

3800 ± 700 gag proteins

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145nm immature virion formed from ~5000 Gag proteins

Arranged in ~80 Å hexagonal lattice

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Immature virion

Tighter hexagonal lattice with 8 nm unit cell.

~5000 gag proteins form a complete shell for a 145 nm virus

Mature virion

Looser hexagonal lattice with 9.6 nm unit cell.

~1200 CA proteins form the conical core, leaving a large number elsewhere

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Virology

University of Heidelberg

University of Oxford

Cornell University

Hans-Georg Kraüsslich

Barbara Müller

Marko Lampe

Bärbel Glass

Marc Johnson

Volker Vogt

Quentin Sattentau

Electron Microscopy

University of Oxford

Max-Planck Institute for Biochemistry

Stephen Fuller

Kay Grünewald

Friedrich Förster

Thomas Wilk

Giulia Zanetti

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