Protein engineering
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LECTURE 8 :. Protein Engineering. Biotechnology; 3 Credit hours Atta- ur - Rahman School of Applied Biosciences (ASAB) National University of Sciences and Technology (NUST). Protein Structure and Function.

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Protein engineering


Protein Engineering

Biotechnology; 3 Credit hours

Atta-ur-Rahman School of Applied Biosciences (ASAB)

National University of Sciences and Technology (NUST)

Protein structure and function
Protein Structure and Function

  • Proteins are biochemical compounds consisting of one or more polypeptides typically folded into a globular or fibrous form, facilitating a biological function.

  • A polypeptide is a single linear polymer chain of amino acids bonded together by peptide bonds between the carboxyl and amino groups of adjacent amino acid residues.

  • The sequence of amino acids in a protein is defined by the sequence of a gene, which is encoded in the genetic code.

Protein engineering1
Protein Engineering

  • The design and construction of new proteins or enzymes with novel or desired functions, through the modification of amino acid sequences using recombinant DNA technology.

  • The sizes and three dimensional conformations of protein molecules are also manipulated by protein engineering.

Protein engineering2
Protein Engineering

  • Proteins are engineered mainly for two reasons:

    • To elucidate the molecular basis of protein structure and function relationships and to understand catalytic mechanism.

    • To alter protein structure and function either rationally or randomly, i.e. protein design.

  • Most ambitious goal of protein engineering is designing a new function in proteins of known structure

What can be engineered in proteins
What can be engineered in Proteins?


  • Thermodynamic Stability

  • Thermal and Environmental Stability (Temperature, pH, Solvent, Detergents, Salt …..)

What can be engineered in proteins1
What can be engineered in Proteins?


  • Binding (Interaction of a protein with its surroundings)

  • How many points are required to bind a molecule with high affinity?

  • Catalysis (a different form of binding – binding the transition state of a chemical reaction)