DC vaccine & Anti-tumor. “ 生命之树 ” 课题组： 孔琰 唐跃 田亚楠 王超 王京 王欠欠. The tumor induced immunity DC vaccine Induce in vitro & proliferation Classification DC vaccine The function of DC in anti-tumor Reinfuse of DC Our opinion. Inhibit formation of tumor vessel. Tolerance. Tumor Ag. DC.
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孔琰 唐跃 田亚楠
王超 王京 王欠欠
VEGFC（血管内皮生长因子）——Depress the differentiation of pre-DC to mature DC
IL-10——Inhibit the expression of co-stimulatory molecules
TGF-ß——Inhibit the activation of specific CTL
Definition: the DC derive from peripheral blood mononuclear cells(PBMCs) are stimulated by peptide antigen,tumor lysates,whole-cell prepration RNA or DNA of tumor.And the reinfuse the DC into the patient as vaccine.
The tumor peptide in HCC is mainly alpha-fetoprotein(AFP)
AFP It has no affection to DC even at high concention.
Targeting, avoid unnecessary immune stimulation.
higher concentration of tumor Ag stimulate the activation of T cell efficiently
There is little confirmed; Specific recognization
Little monoclonal tumor Ag
tumor cell Ag prepared by direct supersonic breakdown or repeated freezing&melting etc. is useful，no desire for Ag recognizing epitope
hard to supply large amount of fluid tissue；
tumor lysate include normal self-Ag,which easily induce self-immune disease after immunization。2Tumor cell lysate-sensitized DC
Unrelated tumor cell
Easily abtaned from biopsy、operation,HCC tissue specimen from；advanced skill to obtain pre-DC via cell culture；hybrid cell can present HCC Ag in the form of MHC pep complex；hybrid cells in vivo can stimulate to form lasing & steady Ag
Selection of hybrided cells
3DC-tumor cell hybrids
DC subcellular structure & other DC hybrids inhense expression of DC
Antigen gene (HCC HBsAg AFP)
Cytokine gene(GM-CSF IL-12)
Chemotactic gene (lymphotactin Lpin)
AFP gene modefied DC vaccine can express
completely endogenously & continuously
reduce autoimmune diseases
The technology is not developed
IL-18 & Survivin(存活素)
Secrete CKs & cause damage
Migrate to lymph node &spleen Self or other APC secrete IL-12
IFN-γ TNF-α GM-CSF IL-3
Until now many cancers can be treated,
B cell lymphoma cancer
1. the most potent APC
2.the only cell can active naïve T cell to elicit primary immune response.
3.play an significant role in anti-tumor
DC attaches at the surface of NK cell and stimulates related receptors ,so these two cells can communicate in the form of neural synapse.