Inducing mutations in paramecia with ultraviolet light
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Inducing Mutations in Paramecia with Ultraviolet Light. Megamecia. Introduction. Model Organism Paramecium Tetraurelia Mutagen Ultraviolet Radiation. Paramecium Tetraurelia. Ultraviolet Radiation. Electromagnetic Spectrum 100 nm – 400 nm range

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Inducing Mutations in Paramecia with Ultraviolet Light

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Inducing mutations in paramecia with ultraviolet light

Inducing Mutations in Paramecia with Ultraviolet Light

Megamecia


Introduction

Introduction

  • Model Organism

    • Paramecium Tetraurelia

  • Mutagen

    • Ultraviolet Radiation


Paramecium tetraurelia

Paramecium Tetraurelia


Ultraviolet radiation

Ultraviolet Radiation

  • Electromagnetic Spectrum

    • 100 nm – 400 nm range

  • Causes pyrimadine dimers to form in DNA (Goodsell, 2001)

    • Pyrimadine dimers may result in the formation of a point mutation.

http://www.uvsciences.com/uvtech.html


Pyrimadine dimers

Pyrimadine Dimers

  • Covalent bond between two adjacent pyrimadine bases (Thyamine, Cytosine)

    • Point Mutation – Transition Mutation

      • Switching of complementary base pairs A-T becomes G-C (Russell, 2010)

http://www.mun.ca/biology/scarr/F16-20,25.jpg

http://www.uvsciences.com/images/dna.jpg


Methods

Methods

  • 500 μL culture samples added to tissue culture tray

  • Samples exposed to UV radiation in a UV chamber.

    • Exposed between 10 and 60 minutes in 10 minute intervals


Methods1

Methods

  • Culture trays wrapped in tin foil immediately after exposure.

    • Paramecia have the ability to repair DNA while exposed to white light

  • Repeated 30 minute exposure with new samples

    • 200 μL klebsiella inoculated wheat media added to each sample after exposure

  • Samples checked periodically for changes

    • Photos taken using a digitial microscope and Applied Vision

    • Measurements done with Image J


Results

RESULTS

  • 30 minutes was the optimum time to keep the cells in UV light

  • Mutant cells were 22% larger in length & 15% larger in width


Results1

RESULTS

MUTANTS

CONTROL


Discussion

DISCUSSION

Other mutations that weren’t able to be cultured


Discussion1

DISCUSSION

  • Experiment could be taken further

    • Analyze DNA to pinpoint where mutation occurred

  • Experimental errors

    • Tissue culture trays should have been checked for cells before exposure to Ultraviolet radiation

  • Ultraviolet radiation successfully caused a mutation


References

REFERENCES

Goodsell, D.S. (2001). The Molecular Perspective: Ultraviolet Light and Pyrimidine Dimers. The Oncologist, 6(3), 298-299. Retrieved from http://theoncologist.alphamedpress.org/cgi/content/full/6/3/298

Russell, P.J. (2010) iGenetics: A Molecular Approach 3rd Ed. San Francisco, CA: Pearson Education, Inc.


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