In silico feedback for in vivo regulation of a gene expression circuit
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In Silico Feedback for In Vivo Regulation of a Gene Expression Circuit. Andreas Milias-Argeitis , Sean Summers, Jacob Stewart-Ornstein, Ignacio Zuleta , David Pincus , Hana El- Samad , Mustafa Khammash , and John Lygeros Nature Biotechnology November 6 th , 2011 Jamal Elkhader

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In Silico Feedback for In Vivo Regulation of a Gene Expression Circuit.

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In silico feedback for in vivo regulation of a gene expression circuit

In SilicoFeedback for In Vivo Regulation of a Gene Expression Circuit.

Andreas Milias-Argeitis, Sean Summers, Jacob Stewart-Ornstein, Ignacio Zuleta, David Pincus, Hana El-Samad, Mustafa Khammash, and John Lygeros

Nature Biotechnology

November 6th, 2011

Jamal Elkhader

20.385 Journal Club II


Feedback control

Feedback Control

  • Regulation of Cell Behavior

  • Feedback Control Important for Regulation

    • Processing measurements in real time

    • Determining inputs

  • Robust regulation can be achieved!


In silico feedback control

In SilicoFeedback Control

  • In silico– Performed on Computer or via Computer Simulation

  • In silicofeedback

    • Computer

    • Real time data

    • Inputs

    • Regulation


Implementation

Implementation

  • S. cerevisiae

  • Light-switchable system (PhyB-PIF3)

  • Transformed cells

    • Grown in Dark

    • PCB


Exposure to light

Exposure to Light

  • Red (650 nm) and Far Red (730 nm) Pulses

  • Input – Light Pulses

  • YFP expression

  • Output - YFP


Results

Results


Results1

Results


Results2

Results


Using a kalman filter

Using a Kalman Filter

  • Real Time Regulation


Summary

Summary


Significance

Significance

  • Enhancement of expression

  • Electronic control over living cells

  • Therapeutics

    • Regulation of expression

    • Regulation of toxic by-products


Questions

Questions?


Light pulse delivery system

Light Pulse Delivery System


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