1 / 13

In Silico Feedback for In Vivo Regulation of a Gene Expression Circuit.

In Silico Feedback for In Vivo Regulation of a Gene Expression Circuit. Andreas Milias-Argeitis , Sean Summers, Jacob Stewart-Ornstein, Ignacio Zuleta , David Pincus , Hana El- Samad , Mustafa Khammash , and John Lygeros Nature Biotechnology November 6 th , 2011 Jamal Elkhader

babu
Download Presentation

In Silico Feedback for In Vivo Regulation of a Gene Expression Circuit.

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. In SilicoFeedback for In Vivo Regulation of a Gene Expression Circuit. Andreas Milias-Argeitis, Sean Summers, Jacob Stewart-Ornstein, Ignacio Zuleta, David Pincus, Hana El-Samad, Mustafa Khammash, and John Lygeros Nature Biotechnology November 6th, 2011 Jamal Elkhader 20.385 Journal Club II

  2. Feedback Control • Regulation of Cell Behavior • Feedback Control Important for Regulation • Processing measurements in real time • Determining inputs • Robust regulation can be achieved!

  3. In SilicoFeedback Control • In silico– Performed on Computer or via Computer Simulation • In silicofeedback • Computer • Real time data • Inputs • Regulation

  4. Implementation • S. cerevisiae • Light-switchable system (PhyB-PIF3) • Transformed cells • Grown in Dark • PCB

  5. Exposure to Light • Red (650 nm) and Far Red (730 nm) Pulses • Input – Light Pulses • YFP expression • Output - YFP

  6. Results

  7. Results

  8. Results

  9. Using a Kalman Filter • Real Time Regulation

  10. Summary

  11. Significance • Enhancement of expression • Electronic control over living cells • Therapeutics • Regulation of expression • Regulation of toxic by-products

  12. Questions?

  13. Light Pulse Delivery System

More Related