Serum Creatine kinase. Description. Creatine kinase (CK), also known as creatine phosphokinase (CPK) or phospho-creatine kinase is an enzyme ( EC 22.214.171.124 ) expressed by various tissues and cell types. Function.
Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author.While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server.
The pattern of isoenzymes found in the plasma may therefore, serve as a means of identifying the site of tissue damage.
In the cells, the "cytosolic" CK enzymes consists of two subunits, which can be either B (brain type) or M (muscle type). There are, therefore, three different isoenzymes:
While mitochondrial creatine kinase is directly involved in formation of phospho-creatine from mitochondrial ATP, cytosolic CK regenerate ATP form ADP.
Clinically, creatine kinase is assayed in blood tests as a marker of:
Normal level at 37 C:
24 -190 u/L in male.
24- 170 u/L in female.
ADP = Adenosine 5'-Diphosphate
ATP = Adenosine 5'-Triphosphate
G-6-PDH = Glucose-6-Phosphate Dehydrogenase
ß-NADP = ß-Nicotinamide Adenine Dinucleotide Phosphate,
ß-NADPH =ß-Nicotinamide Adenine Dinucleotide Phosphate,
6-PG = 6-Phospho-D-Gluconate
CONDITIONS:T = 30°C, A 340 nm.
Serum, heparinised plasma and Edta plasma.
Different CK isoenzymes pattern can be detected by electrophoresis.
Stained with ethidium bromide (EtBR) to Visualize the DNA
DNA is loaded
Screening PCR products to test for the presence of specific DNA sequences