Serum Creatine kinase. Description. Creatine kinase (CK), also known as creatine phosphokinase (CPK) or phospho-creatine kinase is an enzyme ( EC 126.96.36.199 ) expressed by various tissues and cell types. Function.
The pattern of isoenzymes found in the plasma may therefore, serve as a means of identifying the site of tissue damage.
In the cells, the "cytosolic" CK enzymes consists of two subunits, which can be either B (brain type) or M (muscle type). There are, therefore, three different isoenzymes:
While CK-MB (1%). mitochondrial creatine kinase is directly involved in formation of phospho-creatine from mitochondrial ATP, cytosolic CK regenerate ATP form ADP.
Diagnostic Use CK-MB (1%).
Clinically, creatine kinase is assayed in blood tests as a marker of:
Normal level at 37 C:
24 -190 u/L in male.
24- 170 u/L in female.
ADP CK-MB (1%). = Adenosine 5'-Diphosphate
ATP = Adenosine 5'-Triphosphate
G-6-PDH = Glucose-6-Phosphate Dehydrogenase
ß-NADP = ß-Nicotinamide Adenine Dinucleotide Phosphate,
ß-NADPH =ß-Nicotinamide Adenine Dinucleotide Phosphate,
6-PG = 6-Phospho-D-Gluconate
CONDITIONS:T = 30°C, A 340 nm.
Serum, heparinised plasma and Edta plasma.
Different CK isoenzymes pattern can be detected by electrophoresis.
Agarose Gel electrophoresis.
Stained with ethidium bromide (EtBR) to Visualize the DNA
DNA is loaded
Screening PCR products to test for the presence of specific DNA sequences