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Structures of the Purine and Pyrimidine Bases

Structures of the Purine and Pyrimidine Bases. Example of the Structure of a Nucleotide. Base. Nucleoside. Nucleotide. Chemical Structure of a Nucleic Acid. Voet Fig. 3-6. Double Helical Structure of DNA. Note that the strands run antiparallel Note that the helix is 20 Å wide.

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Structures of the Purine and Pyrimidine Bases

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  1. Structures of the Purine and Pyrimidine Bases

  2. Example of the Structure of a Nucleotide Base Nucleoside Nucleotide

  3. Chemical Structure of a Nucleic Acid Voet Fig. 3-6

  4. Double Helical Structure of DNA Note that the strands run antiparallel Note that the helix is 20 Å wide. There are about 10 base pairs per turn of the helix. One turn of the helix is 34 Å The base pairs are 3.4 Å apart Major groove Voet Fig. 3-9

  5. Base Pairing Voet Fig. 23-1

  6. Many proteins associate With DNA through the Major and Minor grooves Major Groove http://www.clunet.edu/BioDev/omm/gallery.htm

  7. Space-filling Model of B-DNA Backbones of the strands are bright green and bright red The bases on the corresponding backbones are lighter green and pink

  8. Helical Structures A-DNA B-DNA Z-DNA Voet Fig. 23-2

  9. Supercoiling of DNA Supercoiled DNA Relaxed DNA

  10. Denaturation of DNA Voet Fig. 23-15

  11. Restriction Endonucleases Voet Fig. 3-18

  12. Restriction Digest Cutting DNA with Restriction Endonucleases followed by Analysis by Gel Electrophoresis B A C A C B

  13. Visualizing DNA Fragments in Gels Voet Fig. 3-20

  14. Southern Analysis Voet Fig. 23-28

  15. Chain Termination Method of DNA Sequencing Voet Fig. 3-23

  16. DNA Sequencing Instruments Fluorescent dye-conjugated derivatives of the Dideoxynucleotides can be used in DNA Sequencing Instruments

  17. Automated Sequencing Example of data obtained from a DNA sequencing reaction using fluorescent (dye-conjugated) derivatives of dideoxynucleotides Voet Fig. 3-25

  18. Polymerase Chain Reaction Amplification of DNA Garrett and Grisham Fig. 13-21 See Voet Fig. 3-32

  19. Plasmids can Serve as Cloning Vehicles (Vectors) Voet Fig. 3-27

  20. Construction of a Recombinant DNA Voet Fig. 3-29

  21. Selection of Transformants Vector DNAs must be capable of replicating in the host cells and must carry a selectable marker

  22. Selection of Transformants of Interest Voet Fig. 3-31

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