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PRELIMINARY RESULTS ON THE SYNTHESIS OF METAL COMPLEX-PNA CONJUGATES PowerPoint PPT Presentation


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Ruprecht-Karls-Universität Heidelberg, Anorganisch-Chemisches Institut Im Neuenheimer Feld 270, D-69120 Heidelberg. E-mail: [email protected] RESULTS

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PRELIMINARY RESULTS ON THE SYNTHESIS OF METAL COMPLEX-PNA CONJUGATES

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Preliminary results on the synthesis of metal complex pna conjugates

Ruprecht-Karls-Universität Heidelberg, Anorganisch-Chemisches Institut Im Neuenheimer Feld 270, D-69120 Heidelberg. E-mail: [email protected]

RESULTS

Coupling of carboxylic acids onto 5’-terminus of PNA was accomplished using an activation mixture of HBTU, HOBT, and DIEA and protected carboxylic acids. Yields were consistently higher then 90 %.

After treatment with different transition metal ions the chemical stability of the modified PNA was determined by MALDI- TOF MS.

Metal-complex PNA conjugates were analysed by MALDI- TOF MS.

ABSTRACTNonenzymatic hydrolysis of the phosphodiester backbone of nucleic acids is an attractive research aim in molecular biology, but the sequence specific hydrolysis of ssDNA was achieved only in one case with a conjugate consisting of a Ce(IV)-complex attached to a DNA-oligonucleotide.[1] The nonenzymatic sequence specific hydrolysis of dsDNA is not reported.Since peptide nucleic acids show interesting binding properties we are focussing on the design of metal-complex-PNAs in a way that allows the generation of combinatorial libraries. Their synthesis and characterization will be discussed.

Here we report on the synthesis of some conjugates of PNA (peptide nucleic acids) (blue) with a varity of poly-

dentate metal- binding fragments (yellow) , which can hydrolyse the phosphodiester bonds of target oligonucleotides (black).

Restriction enzymes that hydrolyse the phosphodiester backbone of DNA play an important role in biological processes and in biotechnology.

These enzymes recognize specific sequences of 4-8 base pairs.

INTRODUCTION

POLYDENTATE LIGANDS ATTACHED TO 5´- TERMINUS OF PNA

Fig. 1:Mechanism of sequence specific cleavage

of polynucleotides with artificial nucleases.

Although artificial restriction enzymes offer unlimited sequence specifity and better chemical stability, only one case is reported in which sequence specific hydrolysis of ssDNA was achieved by a conjugate consisting of a Ce(IV)- complex attached to a DNA- oligonucleotide.[1]

SOLID PHASE SYNTHESIS OF POTENTIAL SEQUENCE SPECIFIC NUCLEASES

PRELIMINARY RESULTS ON THE SYNTHESIS OF METAL COMPLEX-PNA CONJUGATES

Felix Zelder, Andriy A. Mokhir, Roland Krämer, Igor Fritsky

Fig. 2: 1; RCOOH, HBTU, HOBT, DIEA, DMF. 2; TFA/ m-Cresol.

MALDI- TOF MS OF A CONJUGATE OF PNA AND METAL COMPLEX

ACKNOWLEDGEMENTS

This work is supported by Ruprecht-Karls-Universität Heidelberg.

Authors thank Dr. Gross (Ruprecht-Karls-Universität Heidelberg) for his kind support with mass spectrometric measurements.

LITERATURE

[1]M. Komiyama, J. Biochem. 1995, 118, 665- 670.

ZrIV

Fig. 3: MALDI- TOF MS spectra of crude NAE-TTCTTCTTTC [M+H]th+ = 2807,5.

and after treatment with Zr(IV)[M+Zr(IV)-3H th] = 2896,3.

Uncertainty of m/ z = 0. 1%.


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