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    • (NA, nutrient agar)

    • nutrient agar platesNA

    • 30

    • 24-48




70



-



Broth


Slant


    • (Streak-Plate Technique)


    • (NA, nutrient agar)

      • Escherichia coli

      • Bacillus subtilis

      • Staphylococcus aureus


    • IIIIII60~90IV

II

I

III

IV


  • I

  • 60~90III8-10


  • 60~90IIIII5-10

  • 60~90IIIZ


II

III

I

IV

(Streak-Plate Technique)


pH

    • (Streak-Plate Technique)

    • pHpH


    • (NA, nutrient agar)

    • (broth)

    • Escherichia coli


    • Z

    • 453725437


- Spread Plate

    • ml

    • (colony forming unit CFU)

    • CFU30-300


    • (NA, nutrient agar)

    • (broth)

    • L

    • Escherichia coli


    • 10-110-210-310-410-510-610-710-8

    • 100 ml

    • 95%95%


  • 37

    • /0.1 ml/

    • 10-5200

      200/0.1 ml/10-5 = 200 x 106 = 2 x 108


  • (Spread-Plate Technique)

    • 1.

    • 2.


  • 3.

  • 4.


- Gram stain


      • 95%


    • (Crystal violet)

    • (Gram's iodine solution)

    • 95%LPS

    • (safranin)


      • Escherichia coli

      • Bacillus subtilis

      • 16~24

      • 95%


    • ECBS

    • RO


  • RO

  • 95%

  • 45

  • 100


E. coli Gram stain


B. natto Gram stain


-()

    • (disc-agar diffusion)Kirby-Bauer


    • antibiotics

    • ampicillinkanamycinchloramphenicoltetracycline

    • Kirby-Bauer


      • Escherichia coli

        • ampicillin

        • kanamycin


    • amp, kan, tetra

    • 90


  • 37

  • (mm)resistantintermediatesensitive


  • Mueller-Hinton agar

  • ampicillin ()kanamycin ()aminoglycosidetRNA30Schloramphenicol ()50Stetracycline ()30S


E. coli Top10F strain


E. coli Top10F/pUC18 strain


  • (water agar)-cycloheximide,


  • L(water agar)1/10

    • 1 g9 ml1010-1, 10-2, 10-3, 10-4, 10-5

    • 100 ml

    • 95%95%


  • 3~4



  • (Sabourauds medium)


  • 15 ml Lactophenol cotton blue40X


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