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904574


904574


904574

    • (NA, nutrient agar)

    • nutrient agar platesNA

    • 30

    • 24-48


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70


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-


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Broth


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Slant


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    • (Streak-Plate Technique)


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    • (NA, nutrient agar)

      • Escherichia coli

      • Bacillus subtilis

      • Staphylococcus aureus


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    • IIIIII60~90IV

II

I

III

IV


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  • I

  • 60~90III8-10


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  • 60~90IIIII5-10

  • 60~90IIIZ


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II

III

I

IV

(Streak-Plate Technique)


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pH

    • (Streak-Plate Technique)

    • pHpH


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    • (NA, nutrient agar)

    • (broth)

    • Escherichia coli


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    • Z

    • 453725437


Spread plate

- Spread Plate

    • ml

    • (colony forming unit CFU)

    • CFU30-300


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    • (NA, nutrient agar)

    • (broth)

    • L

    • Escherichia coli


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    • 10-110-210-310-410-510-610-710-8

    • 100 ml

    • 95%95%


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  • 37

    • /0.1 ml/

    • 10-5200

      200/0.1 ml/10-5 = 200 x 106 = 2 x 108


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  • (Spread-Plate Technique)

    • 1.

    • 2.


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  • 3.

  • 4.


Gram stain

- Gram stain


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      • 95%


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    • (Crystal violet)

    • (Gram's iodine solution)

    • 95%LPS

    • (safranin)


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      • Escherichia coli

      • Bacillus subtilis

      • 16~24

      • 95%


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    • ECBS

    • RO


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  • RO

  • 95%

  • 45

  • 100


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E. coli Gram stain


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B. natto Gram stain


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-()

    • (disc-agar diffusion)Kirby-Bauer


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    • antibiotics

    • ampicillinkanamycinchloramphenicoltetracycline

    • Kirby-Bauer


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      • Escherichia coli

        • ampicillin

        • kanamycin


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    • amp, kan, tetra

    • 90


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  • 37

  • (mm)resistantintermediatesensitive


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  • Mueller-Hinton agar

  • ampicillin ()kanamycin ()aminoglycosidetRNA30Schloramphenicol ()50Stetracycline ()30S


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E. coli Top10F strain


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E. coli Top10F/pUC18 strain


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  • (water agar)-cycloheximide,


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  • L(water agar)1/10

    • 1 g9 ml1010-1, 10-2, 10-3, 10-4, 10-5

    • 100 ml

    • 95%95%


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  • 3~4


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  • (Sabourauds medium)


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  • 15 ml Lactophenol cotton blue40X


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