Homework: Complete Step 4 (Summary) on Intro to Enzymes worksheet Silent Do Now:

1. Bio 9A/9D: Tuesday, 11.2.10 Title: Introduction to Enzymes! • Homework: • Complete Step 4 (Summary) on Intro to Enzymes worksheet • Silent Do Now: • Silently pass your typed McMush Lab Report forward • (on the Intro to Enzymes handout) What comes to mind when you hear the word enzyme? What do you think an enzyme is? • Today’s Objectives: • Describe what an enzyme does and how it works • Explain why an enzyme’s shape is so important

2. Web-Based Activity:Guided Notes on Enzymes • Go to “LewPort enzymes” (first link on google) http://www.lew-port.com/10712041113402793/lib/10712041113402793/Animations/Enzyme_activity.html • Read aloud the blue text on each slide • Use this info to fill in the Notes column on the back of your worksheet • Then use the animations on the Enzyme Menu to fill in drawings or descriptions in the Evidence column • We will do the first one together… • You will work quietly w/ your assigned partner to complete the rest. • Objectives for Class: • Describe what an enzyme does and how it works • Explain why an enzyme’s shape is so important

3. Lorena Joelle Alerte Olivia Devin Lila Jesse Martin Lennin Bryan Emiliano Victor Daysia Nathy Cassandra Amrita Kenny Naidaliz Imani Yamile Hajar Javier Erick Joshua Martely Beverly

4. Web-Based Activity:Guided Notes on Enzymes • Go to “LewPort enzymes” (first link on google) http://www.lew-port.com/10712041113402793/lib/10712041113402793/Animations/Enzyme_activity.html • Read aloud the blue text on each slide • Use this info to fill in the Notes column on the back of your worksheet • Then use the animations on the Enzyme Menu to fill in drawings or descriptions in the Evidence column • We will do the first one together… • You will work quietly w/ your assigned partner to complete the rest. • Objectives for Class: • Describe what an enzyme does and how it works • Explain why an enzyme’s shape is so important

5. Bio 9A/9D: Wednesday, 11.3.10 Title: ENZYMES Homework: • Now: Take out Intro to Enzyme worksheet • Tonight: Starch Digestion Pre-Lab Questions (pages 2-3 #1-6) Silent Do Now: (in NB w/ title & date) Describe or define each of the following terms. What does each one mean to you? If you’re not sure, write what you think. What questions do you have about them? How do these terms relate to each other? • Chemical reaction • Enzyme • Reactant • Product You should be writing silently for the entire Do Now time.

6. Today’s Objectives & Agenda • Objectives: • Describe and illustrate chemical reactions, reactants, products, and enzymes • Explain how enzymes work to catalyze reactions • Use a knowledge of enzymes to predict what will happen in tomorrow’s lab • Agenda: • Notes: Vocabulary & Example • Pre-Lab for tomorrow’s lab • Start HW

7. Chemical Reaction Vocabulary: • Chemical Reaction: • Process that changes or transforms one substance into another • Either to build something from smaller pieces – Synthesis • Or to break something down – Decomposition • Ex: glucose + O2 CO2 + H2O + ATP energy + heat • Enzyme: • A protein that speeds up chemical reactions in living things (the “catalysts” in our cells and bodies) • Objectives for Class: • Describe and illustrate chemical reactions, reactants, products, and enzymes • Explain how enzymes work to catalyze reactions • Use a knowledge of enzymes to predict what will happen in tomorrow’s lab

8. Chemical Reaction Vocabulary continued: • Reactants: • the starting materials in a chemical reaction • (also known as substrates) • Products: • substances that are produced in the chemical reaction (the end result) • Objectives for Class: • Describe and illustrate chemical reactions, reactants, products, and enzymes • Explain how enzymes work to catalyze reactions • Use a knowledge of enzymes to predict what will happen in tomorrow’s lab

9. Example: Lipid Digestion The enzyme lipase helps to break down lipids into their building block molecules (fatty acids and glycerol) Enzyme – Reactant (aka Substrate) – Products – Lipase Lipids Substrate/ Reactant Products Fatty acids and glycerol Lipase Lipids Fatty acids + glycerol Enzyme helps substrate turn into products • Objectives for Class: • Describe and illustrate chemical reactions, reactants, products, and enzymes • Explain how enzymes work to catalyze reactions • Use a knowledge of enzymes to predict what will happen in tomorrow’s lab

10. Example: Lipid Digestion Which enzyme is best structured to help break down lipids? Why? 1 2 3 • Objectives for Class: • Describe and illustrate chemical reactions, reactants, products, and enzymes • Explain how enzymes work to catalyze reactions • Use a knowledge of enzymes to predict what will happen in tomorrow’s lab

11. Example: Lipid Digestion Which enzyme is best structured to help break down lipids? Why? The SHAPE of an enzyme must match the SHAPE of its specific substrates! 2 • Objectives for Class: • Describe and illustrate chemical reactions, reactants, products, and enzymes • Explain how enzymes work to catalyze reactions • Use a knowledge of enzymes to predict what will happen in tomorrow’s lab

12. Enzyme Lab Preview:Starch Digestion • Background Info… • Procedure Summary… • Work w/ partner to answer pre-lab questions #1-6 • HW: Finish the pre-lab questions! Starch & Water & Enzyme (salivary amylase) Starch & Water • Objectives for Class: • Describe and illustrate chemical reactions, reactants, products, and enzymes • Explain how enzymes work to catalyze reactions • Use a knowledge of enzymes to predict what will happen in tomorrow’s lab

13. Bio 9A/9D: Thursday, 11.4.10Title: Enzyme Lab: Starch Digestion • Homework: Enzyme Lab Analysis Questions (typed or neatly written on separate paper) • Silent Do Now: (in notebook w/ title & date) • In 1-2 sentences, explain what this chemical equation means: STARCHGLUCOSE • Identify the reactant, product, and enzyme in this reaction. • Which two substances will we add to the test tube at the beginning of our experiment? • Which substance will be in the test tube at the end of the experiment that wasn’t there at the beginning? salivary amylase

14. Today’s Objectives & Agenda • Today’s Objectives: • Identify the reactant, product, and enzyme in a chemical reaction • Predict how an enzyme affects the speed of a chemical reaction • Use proper lab procedures to test how saliva enzymes affect the speed at which starch breaks down into glucose • Today’s Agenda: • Review Do Now & Lab Procedures • Do the lab!

15. Enzyme Lab: Starch DigestionSTARCH GLUCOSE salivary amylase • Procedure • Prediction: • Results: How will we know? *Stir the starch well before using it! Starch & Water Starch & Water & Enzyme (salivary amylase) 1 2 Which tube will contain glucose after 10 minutes? Fill in the Prediction row on your lab table right now. Benedict’s test

16. Enzyme Lab: Starch DigestionSTARCH GLUCOSE salivary amylase • Safety: • Goggles on! • Be careful around hot plates! • Stay at your own lab station, do not talk across the room • No eating/drinking • Clean Up: • Pour test tubes into waste container • Rinse test tubes in sink • Put tray containing all materials on side counter • Turn off hot plates if everyone at your table is done • If finished early: discuss Analysis Question #2 with your partner

17. Lorena Joelle Alerte Olivia Devin Lila Jesse Martin Lennin Bryan Emiliano Victor Daysia Nathy Cassandra Amrita Kenny Naidaliz Emiliano Yamile Hajar Javier Erick Joshua Martely Beverly

18. 9A – New Seats You are sitting with your partner from the Enzyme Lab Lorena Joelle Martin Jesse Lennin Bryan Amrita Kenny Martely Devin Lila Beverly Victor Daysia Nathy Cassandra Naidaliz Emiliano Javier Erick Joshua Yamile Olivia Hajar

19. Bio 9A: Monday, 11.8.10Title: Enzyme Lab Extension • Homework: Type up the Background Info and Purpose & Hypothesis sections of your Denaturing Enzymes lab report (see rubric for details) • Silent Do Now: Complete questions 1 and 2 on the Denaturing Enzymes packet. • Today’s Objectives: • Define and illustrate denaturing and explain how it affects the function of enzymes • Develop a specific experimental question and hypothesis related to denaturing enzymes

20. Quick Review: Enzyme-Catalyzed Reactions Substrate = Reactants of a Chemical Reaction “Active Site” – where the substrate bonds to the enzyme Enzyme = Protein that “Speeds Up” a Chemical Reaction Enzymes are a “catalyst” This shows the reaction happening!!! Products = Stuff Produced from a Chemical Reaction “Enzyme – Substrate Complex” • Objectives for Class: • Define and illustrate denaturing and explain how it affects the function of enzymes • Develop a specific experimental question and hypothesis related to denaturing enzymes

21. Denaturing Enzymes When an enzyme is denatured, it… • Becomes misshapen and deformed • Loses its original shape • Becomes unable to speed up the reaction it is designed for, because its shape no longer matches its substrate

22. Design Your Own Experiment • Work with your partner to complete pages 2 and 3 of the Denaturing Enzymes Lab Packet • Purpose and Hypothesis • Background Information • If you finish early, move on to the Procedure section (page 4) • HW: Write up the Background Info and Purpose & Hypothesis sections of the lab report • See rubric for details • Type if possible (and bring electronic copy on Wed.) Hand in Analysis Questions at the end of today’s class

23. Background Info Brainstorm • Enzyme • Denature • Salivary amylase • Heat or pH (acid/base) (depending on your question) • Glucose • Substrates • Products • Chemical reaction • Starch

24. Bio 9A: Wednesday, 11.10.10Title: Denaturing Enzymes Lab (Day 2) • Homework: Write (type if possible) the Procedure section of your Denaturing Enzymes lab report (see rubric for details) • Silent Do Now: (in NB w/ title and date) • Summarize what it means for an enzyme to be denatured. Is this generally a good thing or a bad thing? Why? • Describe the experiment you are planning. How will you denature the enzyme? What do you think will happen? • Today’s Objectives: • Write a clear, specific, and replicable procedure for carrying out an experiment relating to denaturing enzymes • Design a clear data table for recording the results of an experiment

25. Revised Work Policy • You must turn in the original assignment and rubric, along with your revisions • Posters: Redo OR just add missing info • Lab Reports: Type revisions and reprint • Staple them all together • Revisions due FRIDAY if you want them to show up on your 1st quarter report card • Otherwise, they still count towards raising your 1st quarter grade but won’t show up until the next report card

26. Denaturing Enzymes Lab:Procedure & Data Table • Work with your partner to complete pages 4 and 5 of the Denaturing Enzymes Lab Packet • Plan your Procedure by answering the questions • Get Dickson’s approval • Write the Steps and Materials for your procedure • Write Materials List on clipboard on kidney • On page 6: Create your data table • HW: Write up the Procedure section of the lab report • See rubric for details • Type if possible (and bring electronic copy on Friday) • Friday: Do the experiment, then laptops for typing!

27. Lorena Joelle Javier Erick Joshua Devin Imani Lila Jesse Martin Lennin Bryan Victor Daysia Nathy Cassandra Amrita Kenny Naidaliz Emiliano Yamile Hajar Martely Beverly Olivia Alerte

28. Bio 9A: Friday, 11.12.10Title: Denaturing Enzymes Lab (Day 3) • Homework: Finish typing the Lab Report (all sections) for the Denaturing Enzymes Lab • Silent Do Now: • Take out the Denaturing Enzymes Lab packet • On page 6, create a data table for recording the results of your experiment. Remember to include: • Space to record results from all of your test tubes • Space to record observations as well as +/- results

29. Today’s Objectives & Agenda • Today’s Objectives: • Design a clear, specific, and replicable procedure and a clear datatable for your experiment • Carry out your experiment • Write a formal lab report • Agenda: • Procedure Checklist (do this silently while Dickson sets up groups) • Get final procedure approved • Gather materials • Carry out experiment • Discuss results w/ Ms. Dickson • Type Lab Report (sit at front desks)

30. 9A – New Seats for today Lorena Lila Javier Jesse Lennin Yamile Joelle Bryan Hajar Kenny Beverly Alerte Victor Martely Martin Amrita Devin Emiliano Cassandra Joshua Olivia Daysia Erick Naidaliz Imani Nathy

31. Bio 9A: Monday, 11.15.10Title: Denaturing Enzymes Lab Wrap-Up • Homework: Revise your lab report based on today’s peer editing. Final draft due tomorrow, with rubric! • Silent Do Now: • Take out your lab report • On the top, write: • What is one thing you think you have done well in this rough draft? • What is one thing you think you could improve? What would you most like feedback/suggestions on?

32. Today’s Objectives & Agenda • Today’s Objectives: • Compare and contrast experimental results between different groups, and draw class-wide conclusions • Use the lab report rubric and peer editing sheet to give and receive helpful feedback on the Denaturing Enzymes lab report • Agenda: • Groups report out on results • Peer editing of lab reports

33. In your notebook set up a place for Denaturing Enzyme Lab Class Results • How does HEAT affect enzymes? • How does extreme pH affect enzymes?

34. Class Results:How does HEAT affect enzymes? + = positive result (glucose was present, reaction occurred) – = negative result (no glucose present, reaction didn’t occur)

35. In your notebook set up a place for Denaturing Enzyme Lab Class Results • How does HEAT affect enzymes? • Boiling enzymes denatures some enzymes but not all • Maybe needed to boil them longer • Heat doesn’t make enzymes completely useless, just less effective • How does extreme pH affect enzymes? • Lemon juice (acid) and bleach (base) denature enzymes • Hydrochloric acid is not consistent but using more works better • Maybe household items work better than pure acids?

36. Class Results:How does pH affect enzymes? + = positive result (glucose was present, reaction occurred) – = negative result (no glucose present, reaction didn’t occur)

37. Today’s Objectives & Agenda • Today’s Objectives: • Compare and contrast experimental results between different groups, and draw class-wide conclusions • Use the lab report rubric and peer editing sheet to give and receive helpful feedback on the Denaturing Enzymes lab report • Agenda: • Groups report out on results • Peer editing of lab reports – this is silent individual work time • Switch lab reports with your assigned partner. • Read the lab report and evaluate each section (just circle boxes, don’t give points/grades) • Fill out the feedback form on the back of the rubric. • Last 5 min. of class = time for quiet partner discussion

38. Bio 9A: Tuesday, 11.16.10Title: Quiz Review: Carbon Compounds & Enzymes • Homework: Study for tomorrow’s quiz • Silent Do Now: (in NB w/ title and date) • Take out your lab report and rubric and put it on the corner of your desk • Take out your assignment sheet and find the study guide on the back. • Answer questions 1 and 2 in your notebook (refer to notes from 10/20/10 if you can’t remember)

39. Quiz Review: Carbon Compounds & EnzymesToday’s Objectives & Agenda • Today’s Objectives: • Review important information for tomorrow’s quiz • Identify specific topics that require additional review • Agenda: Work individually or with a partner to quietly complete the study guide • If you want to work with a partner, raise your hand and ask. • Write answers to all study guide questions in your notebook. • If noise levels get too loud… 3rd warning = silent individual work • Raise your hand if you need to move across the room • Work silently or talk quietly with your partner only

40. Bio 9A: Wednesday, 11.17.10Title: Quiz: Carbon Compounds & Enzymes • Homework: Read Section 38.2 and complete the reading guide • Do Now: • Clear your desks and get quiet for the quiz • When Finished with the Quiz: • Get a textbook and start the HW • Today’s Objectives: • Demonstrate what you know about carbon compounds and enzymes on the quiz