Che 440 540 2d nmr
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Che 440/540 2D-NMR. http://www.ch.ic.ac.uk/ectoc/echet96/papers/107/index.htm. Prototype Pulse Sequence for a 2D-NMR Experiment. 90 o pulse. 90 o pulse. Acquire ( t 2 seconds).  t 1 (microseconds). Relaxation delay. Origin of the Second Dimension. Stacked plot of 22

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Che 440/540 2D-NMR

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Che 440 540 2d nmr

Che 440/540 2D-NMR

http://www.ch.ic.ac.uk/ectoc/echet96/papers/107/index.htm


Che 440 540 2d nmr

Prototype Pulse Sequence for a 2D-NMR Experiment

90o pulse

90o pulse

Acquire

(t2 seconds)

t1

(microseconds)

Relaxation

delay


Che 440 540 2d nmr

Origin of the Second Dimension

Stacked plot of 22

spectra of acetone

Resulting interferogram

Projection of (a)


Che 440 540 2d nmr

Fourier transform of a series of interferograms creates the second dimension.


Che 440 540 2d nmr

A Simulated 1H-1H Correlated Spectroscopy (COSY) Spectrum


Che 440 540 2d nmr

Actual COSY Spectrum of Isobutanol

A

B

A, B

C

B,C


Che 440 540 2d nmr

COSY Spectrum of Ipsenol


Che 440 540 2d nmr

Basics of HETCOR and HMQC

  • Heteronuclear correlated (HETCOR) and heteronuclear multiple quantum coherence (HMQC) spectroscopy show direct correlations between hydrogens and their directly bonded carbons.

  • In HETCOR the signal is detected by observing 13C, and in HMQC by observing 1H. The HMQC experiment is shorter, but the pulse sequence is more complicated.

  • Both give the same information: one-bond 1H-13C correlations


Che 440 540 2d nmr

A simulated HMQC spectrum

1H chemical

shifts

13C chemical

shifts

no correlation


Che 440 540 2d nmr

HMQC Spectrum of Ipsenol

diastereotopic CH2’s


Che 440 540 2d nmr

COSY Spectrum of Strychnine


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