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1. 4. mAU. 175. 2. 6. 5. 150. 3. 7. 1 4-Acetamidophenol 2 Caffeine 3 2-Acetamidophenol 4 Acetanilide 5 Acetylsalicylic Acid 6 Phenacetin 7 Salicylic Acid. 125. 100. 75. 50. 25. 0. 0. 0.25. 0.5. 0.75. 1. 1.25. 1.5. 1.75. 2. min. mAU. 1. 175. 4. 150. 2.

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Min

1

4

mAU

175

2

6

5

150

3

7

1 4-Acetamidophenol

2 Caffeine

3 2-Acetamidophenol

4 Acetanilide

5 Acetylsalicylic Acid

6 Phenacetin

7 Salicylic Acid

125

100

75

50

25

0

0

0.25

0.5

0.75

1

1.25

1.5

1.75

2

min

mAU

1

175

4

150

2

6

125

5

7

100

3

3.5 um

75

50

25

LC Conditions

Column: SB-C18, 4.6 x 30 mm

Detector: 254 nm

Injector: 1 ul,

Mobile Phase: 1% Formic Acid

Acetonitrile

(82:18)

Flow: 2.0 ml/min

0

0

0.25

0.5

0.75

1

1.25

1.5

1.75

2

min

mAU

1

4

175

2

6

150

5

3

7

5 um

125

100

75

50

25

0

0

0.25

0.5

0.75

1

1.25

1.5

1.75

2

min

Aumento della Risoluzione in funzione del Particle Size

1.8 um


Min

Piccola quantità di particelle più grandi

Meno particelle

più piccole


Min

0

1

2

3

4

5

6

7

min

Aumento della Risoluzione in funzione del Particle Size

5µm: Rs(4,5) = 3.63

Peak Width, 5s (9) = 0.103

+ 50% Resolution (Theory: + 67%)

+ 125% Efficiency (Theory: + 177%)

1.8µm:Rs(4,5) = 5.45

Peak Width 5s(9) = 0.075

Rs = +50%

Colonna SB-C18 RRHT 4.6 x150mm

Massima lunghezza


Min

1

2

3

Rs(1,2) = 3.1

4

0.5

1

1.5

2

2.5

1100 HT + 1.8um RRHT :

  • Reduction of analysis time from 30 min to 2 min

  • Speed: 14x faster than 4.6 x 250mm, 5um

  • Time saving: 92%

  • Resolution: -35%

AUMENTO DELLA VELOCITA’ D’ANALISI CON HPLC 1100

1

2

3

4.6 x 250 mm, 5 mm

1 mL/min

Rs(1,2) = 4.8

29.65

N=21848

4

N=22680

0

5

10

15

20

25

30

min

1

2

3

Rs(1,2) = 3.5

12.71

4.6 x 100 mm, 3.5 mm

1 mL/min

4

N=11691

1

0

5

10

15

20

25

30

min

.

2

3

N=6568

Rs(1,2) = 3.3

4.6 x 30 mm, 1.8 mm

1 mL/min

4.15

4

N=6104

0

5

10

15

20

25

30

min

N=6463

4.6 x 30 mm, 1.8 mm

2 mL/min

2.09

N=6460

0

5

10

15

20

25

30

min


Min

Ultra-fast analysis in 26 sec

4 peak width ~ 0.6 sec

< 50 sec cycle time

7 analgesics separated in 21 sec

for peaks 1,2,3

Total time Injection-to-Injection

Higher flow rate:

higher speed – same resolution – higher sensitivity/precision

Sample: 7 Analgesics

Column: Zorbax RRHT SB-C18, 1.8 um, 4.6 x 30 mm

2 ml/min – 90bar

Rs2,1 = 2.2

Rs7,6 = 2.2

3 ml/min – 133bar

4 ml/min – 181bar

Rs2,1 = 2.1

Rs7,6 = 2.2

2x faster than 2ml/min without loss in resolution!

Speed


Min

HPLC, 40°C

SB-C18

4.6 x 150mm, 5µm

1.20ml/min, 40°C

Analysis Time = 11min

min

0

2

4

6

8

10

RRHT SB-C18

2.1mm x 50mm 1.8µm

1.00ml/min, 40°C

Analysis Time= 1.1min

RRLC, 40°C

10x faster

0.2

0.4

0.6

0.8

1

min

0

RRHT SB-C18

2.1mm x 50mm 1.8µm

2.40ml/min, 95°C

Analysis Time: 0.4min

RRLC, 95°C

27x faster

0.2

0.4 min

0.6

0

Rapid Resolution HT Columns – Up to 20X Faster

  • Up to 20x faster than conventional HPLC

  • With same or better performance (resolution, precision, sensitivity, carry over)

  • Compliant with strictest regulatory performance requirements

>20X faster


Min

4

5

0

1

2

3

4

5

6

7

min

NON SOLO VELOCITA’ MA ANCHE EFFICIENZA

5µm: Rs(4,5) = 3.63

Peak Width, 5s (9) = 0.103

+ 50% Resolution (Theory: + 67%)

+ 125% Efficiency (Theory: + 177%)

1.8µm:Rs(4,5) = 5.45

Peak Width 5s(9) = 0.075

Rs = +50%

Colonna SB-C18 RRHT 4.6 x150mm

Massima lunghezza


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