Dna fingerprinting
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DNA Fingerprinting. DNA Fingerprinting. In DNA Fingerprinting, the DNA of an organism is cut up into fragments using restriction enzymes producing a large number of short fragments of DNA Because no two individuals have identical DNA, no two individuals will have the same length fragments.

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DNA Fingerprinting

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Dna fingerprinting

DNA Fingerprinting


Dna fingerprinting1

DNA Fingerprinting

  • In DNA Fingerprinting, the DNA of an organism is cut up into fragments using restriction enzymes producing a large number of short fragments of DNA

  • Because no two individuals have identical DNA, no two individuals will have the same length fragments


Gel electrophoresis

Gel Electrophoresis

  • Gel electrophoresis is a technique used to separate the DNA fragments according to their size

  • The fragments are placed in wells on a sheet of gelatin, and an electric current is applied to the sheet


Dna fingerprinting

  • DNA is negatively charged and will move in an electric field toward the positive pole


Dna fingerprinting

  • The smallest fragments will move the fastest because they are able to move through the pores in the gelatin faster

  • Bands will be produced on the gelatin where the fragments accumulate


Dna fingerprinting

  • DNA bands must be stained to make them visible


Dna fingerprinting

  • Used to analyzegenes to test for presence of different alleles

    • If a person was homozygous for a trait, the DNA would be cut by the same restriction enzyme in the same place because alleles are the same

Allele from Dad

Allele from Mom


Dna fingerprinting

  • If a person was heterozygous for a trait, the DNA would be cut by the same restriction enzyme in different places because the alleles are different

Allele from Dad

Allele from Mom


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