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National Biology. Protein and enzymes c: Enzyme specificity. Recap. Draw lolly stick/name/number out of hat to select one person in class to recap what was talked about/learned in the last lesson Warning! Be on your guard it may be your turn next!!. What are we learning today?.

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National biology

National Biology

Protein and enzymes c: Enzyme specificity


Recap

Recap

  • Draw lolly stick/name/number out of hat to select one person in class to recap what was talked about/learned in the last lesson

  • Warning! Be on your guard it may be your turn next!!


What are we learning today

What are we learning today?

We are learning to:

  • Give examples of enzyme controlled reactions to illustrate enzyme specificity

    Success criteria:

  • An enzyme is specific to one substrate

  • Give examples


What does enzyme specificity mean

What does enzyme specificity mean?

  • The substance an enzyme acts on is the SUBSTRATE.

  • What is made in the reaction is called the PRODUCT.


Experiment 2

Experiment 2

  • Aim: to find out what is meant by enzyme specificity using enzymes and various substrates

D

Catalase and starch test for sugar using clinistix

C

Catalase and hydrogen peroxide test for oxygen using glowing splint

A

Amylase and starch test for sugar using clinistix

B

Amylase and hydrogen peroxide test for oxygen using glowing splint


Experiment conclusions

Experiment conclusions

  • Catalase only acts on hydrogen peroxide

  • Amylase only acts on starch

  • Enzymes are SPECIFIC

    • This means that they only work on one substrate

    • Enzymes are complimentary in shape to their substrate


Enzymes are specific

water

oxygen

Enzymes are Specific

  • KEYS are SPECIFIC to LOCKS

  • ENZYMES are SPECIFIC to SUBSTRATES

hydrogen

peroxide

catalase


Enzymes are specific1

water

oxygen

catalase

catalase

Enzymes are Specific

  • Catalase (enzyme) will only act on hydrogen peroxide (substrate)

hydrogen

peroxide

Starch

No reaction

No products formed


Enzymes are specific2

water

oxygen

catalase

Enzymes are Specific

  • Only catalase will catalyse the breakdown of hydrogen peroxide

hydrogen

peroxide

hydrogen

peroxide

No reaction

No products formed

amylase


Enzymes are specific3

water

oxygen

catalase

Enzymes are Specific

  • Remember:

    • Enzymes are specific because of their shape.

hydrogen

peroxide

hydrogen

peroxide

No reaction

No products formed

amylase

The shape of amylase is different to catalase so it will not catalyse the reaction.


The active site

The Active Site

  • Where a substrate attaches to an enzyme is called the active site

  • The active site is where the reaction occurs

  • The active site is complimentary to the shape of the substrate

  • The amino acid sequence determines the shape of the active site


National biology

bbcbitesize Standard Grade Biology


Summary

Summary

  • Exit ticket

  • Draw a diagram (not like one you have seen today) to show what you understand about enzyme specificity


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