Keeping a laboratory notebook. good. ^. A laboratory notebook is a record of both physical and mental activity. Laboratory data include tangible data such as gels, scans of peaks, photographs, and computer printouts as well as intangibles such as quantitation, observations and conclusions.
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A laboratory notebook is a record of both physical and mental activity.
Laboratory data include tangible data such as gels, scans of peaks, photographs, and computer printouts as well as intangibles such as quantitation, observations and conclusions.
All of this data are important to preserve and organize!
Paper vs. Digital
-data storage potential
Installed vs. Online
What goes in a notebook?
General Aspects of Dated Entries:
Hypothesis or Goal: Brief statement of purpose
How: Protocols, calculations, reagents, equipment
All that happens (planned or unplanned)
Raw experimental data (primary data)
Pasted in information or reference to data location
Processing of raw data, graphs, Interpretations (secondary data)
Ideas for future experiments
The devil is in the details
Study: Comparative Genomics of wild vs. genetically-modified strawberries
Wild Strawberry DNA extraction
weight of strawberry extracted = 3 g
heavy duty ziploc bag
10 mL DNA extraction buffer (soapy, salty water)
50 mL tube
20 mL ethanol
One wild strawberry was manually mashed in a ziploc bag. 10mL of extraction buffer was added to the bag and again the strawberry was mashed for 1 min. Collected supernatant and measured DNA.
Nanodrop estimate = 12 ng of DNA
Sample stored in DNA extracts box at -20 deg C until all samples to be analyzed are obtained
Q & A (Think-Pair-Share)
Was all information recorded? What is missing?
Why should it be included?
How confident are you in using this material?
How confident are you in repeating the extraction for the GM strawberry?
Notebook Ethics “Notethics?”
Do a search for “Electronic Lab Notebook,”“ELN,” or “Digital Lab Notebook” and you will be flooded with options
Word- notebook layout
Word- notebook layout mode
Wordpress and other blog sites….
Guidelines For Recordkeeping in the Intramural Research Program at the NIH, 2008
Writing the Laboratory Notebook, Kanare, ACS 1985
Linus Pauling Research Notebooks, http://osulibrary.orst.edu/specialcollections/rnb/index.html