1 / 12

Work Plan to do list:

Work Plan to do list:. a table merging work plan dates and mission clarification table timelines for the individual assays a diagram of the Endocrine Disruptor Program activities and validation process. Inutero Lactational Assay. Prevalidation Study

arleen
Download Presentation

Work Plan to do list:

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript


  1. Work Plan to do list: • a table merging work plan dates and mission clarification table • timelines for the individual assays • a diagram of the Endocrine Disruptor Program activities and validation process

  2. Inutero Lactational Assay • Prevalidation Study • EPA will develop a prevalidation study plan based on protocol C in the DRP • The study plan will identify several chemicals and recommended dose levels with justification for choices made • EPA will e-mail the study plan to the EDMVS for a 2-week review and schedule a conference call to discuss comments

  3. IUL Assay (cont) • DRP • The EDMVS will submit comments for incorporation into the final DRP • A table listing the endpoints of interest and predicted responses to agents of known modes of action (a qualitative prediction model) will be included • A chart comparing protocols A,B, and C will be included.

  4. Pubertals: To-Do List

  5. Recommendation on documenting choice of strain/stock

  6. Consider standardizing • vehicle • diet • water • dose-setting • light cycle • definitions and techniques • VO and PPS, including how measurements are to be made • training video for techniques of concern

  7. Multi-dose study • Add weak thyroid chemical if possible

  8. Multi-chemical study • Include small organ weights • add/substitute chemicals as discussed

  9. Other studies to consider • strain differences in onset of puberty • strain differences in sensitivity (e.g., adding Long-Evans rats to multi-dose study) • … but don’t get too caught up in strain issues (see recommendation)

  10. Check literature for • PND of formation of blood/testis barrier in rats

  11. Other items (not specific to pubertals) • Update table of chemicals used in EDSP studies with • ER/AR binding study chemicals • adult male chemicals • corrections • Choose a core set of chemicals to include across assays • Provide EDMVS with rationale for doses when presenting studies

  12. Other items (continued) • Include systemic toxicants that are expected to be non-endocrine-active, if possible, in assays. • Find a more humane way for sacrifice of culls, if possible • Code chemicals in prevalidation studies if possible

More Related