Trichophyton
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Trichophyton

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Trichophyton

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1. Trichophyton The thiamin requirement test: T. tonsorans grows poorly on a chemically defined medium poor in thiamin (Trichophyton Agar #1) and very well on a thiamin enriched medium (Trichophyton Agar #4) T. rubrum and T. mentagrophytes grow equally well on both media ? no elevated thiamin requirement

2. 298. T. tonsurans has a partial requirement for thiamine. Note the enhanced growth on Trichophyton No.4 agar (Difco) containing thiamine298. T. tonsurans has a partial requirement for thiamine. Note the enhanced growth on Trichophyton No.4 agar (Difco) containing thiamine

3. Trichophyton Microscopic exam of cultures Most Trichophyton species produce numerous globose to pyriform microconidia (not septate) in two primary arrangements Along the hyphae likes birds on a telephone wire (en thyrse) or In large clusters (en grappe)

4. Trichophyton Microscopic exam of cultures Microconidia of T. tonsorans tend to vary in size Macroconidia are scarce in this genus If present they are pencil or club shaped (clavate) and multiseptate with thin smooth walls

5. Conidia of Trichophyton 297. Microscopic morphology of T. tonsurans. The hyphae are relatively broad, irregular and much branched with numerous septa. Numerous characteristic microconidia varying in size and shape from long-clavate to broad-pyriform are borne at right angles to the hyphae, which often remain unstained by lactophenol cotton blue (slide 294, 295). Very occasional smooth, thin-walled, irregular, clavate macroconidia may be present in some cultures (slide 296). Numerous swollen giant forms of microconidia and chlamydoconidia are produced in older cultures (slide 297).297. Microscopic morphology of T. tonsurans. The hyphae are relatively broad, irregular and much branched with numerous septa. Numerous characteristic microconidia varying in size and shape from long-clavate to broad-pyriform are borne at right angles to the hyphae, which often remain unstained by lactophenol cotton blue (slide 294, 295). Very occasional smooth, thin-walled, irregular, clavate macroconidia may be present in some cultures (slide 296). Numerous swollen giant forms of microconidia and chlamydoconidia are produced in older cultures (slide 297).

6. Differential Characteristics of Selected Trichophyton

7. Epidermophyton floccosum The genus Epidermophyton is comprised of only one species: E. floccosum Colonies of E. floccosum are typically fluffy and have an off-yellow color described as khaki green

8. Epidermophyton floccosum 151. E. floccosum on mycobiotic agar. Colonies are usually greenish-brown or khaki coloured with a suede-like surface, raised and folded in the centre, with a flat periphery and submerged fringe of growth.151. E. floccosum on mycobiotic agar. Colonies are usually greenish-brown or khaki coloured with a suede-like surface, raised and folded in the centre, with a flat periphery and submerged fringe of growth.

9. Epidermophyton floccosum Diagnostic Microscopic features: Club shaped smooth thin-walled macroconidia Can have multiple macroconidia emanating from one conidiophore E. floccosum produces no microconidia

10. E. floccosum Conidia 154. Microscopic morphology of E. floccosum showing characteristic smooth, thin-walled macroconidia, which are often produced in, clusters growing directly from the hyphae. No microconidia are formed.154. Microscopic morphology of E. floccosum showing characteristic smooth, thin-walled macroconidia, which are often produced in, clusters growing directly from the hyphae. No microconidia are formed.



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