Denaturation temperatuare gradient polymerase chain reaction by product
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Denaturation temperatuare gradient-polymerase chain reaction: by-product?. Ji Youn Lee Cell and microbial engineering laboratory Seoul National University. Let ’ s talk about DTG-PCR. A newly invented tool by modifying the typical PCR for solving traveling salesman problem

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Denaturation temperatuare gradient-polymerase chain reaction: by-product?

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Denaturation temperatuare gradient polymerase chain reaction by product

Denaturation temperatuare gradient-polymerase chain reaction: by-product?

Ji Youn Lee

Cell and microbial engineering laboratory

Seoul National University


Let s talk about dtg pcr

Let’s talk about DTG-PCR

  • A newly invented tool by modifying the typical PCR for solving traveling salesman problem

  • More amplification of DNA strands with lower melting temperature

  • Is it True?

  • Does it operate Well?

  • Is it Worth?


It s time to study dtg pcr

It’s time to study DTG-PCR

  • Experimental part

  • Modeling and simulation part

  • Make use of the published ones

    : Competitive PCR

  • Addition of the ssDNA distribution profile with temperature


Let s think about it

Let’s think about it!


Experimental considerations

Experimental considerations

  • DNA strands with different Tms

    • Same length, different Tm

    • or Different length, different Tm

    • or Different length, same Tm… etc.

  • Experiments

    • Mix the strands (two or more)

    • Perform DTG-PCR in various conditions

    • Analysis: TGGE, DGGE or graduate PCR


Theoretical considerations

Theoretical considerations

  • Studies up to date

    • Competitive PCR

    • Quantitative PCR

    • Internal standards and products

    • Anal Biochem (1993)

      • Quantitative PCR: Theoretical considerations with practical implications

      • Replication efficiency E (product and standard)


Denaturation temperatuare gradient polymerase chain reaction by product

  • NAR (1996)

    • A mathematical model and a computerized simulation of PCR using complex templates

    • Probability calculation

  • PNAS (1996)

    • Efficiency of DNA replication in the PCR

  • NAR (1997)

    • A coalescent approach to the PCR

  • NAR (1998)

    • Modeling and analysis of competitive RT–PCR


Denaturation temperatuare gradient polymerase chain reaction by product

  • JTB (1997)

    • Enzymological considerations for a theoretical description of the quantitative competitive PCR

    • Michaelis-Menten equation (enzyme kinetics)

  • JTB (1997)

    • Theoretical description of the PCR

  • JTB (1999)

    • Polymerase chain reaction: Markove process approach

  • Bioinformatics (2001)

    • Virtual PCR


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