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Denaturation temperatuare gradient-polymerase chain reaction: by-product?. Ji Youn Lee Cell and microbial engineering laboratory Seoul National University. Let ’ s talk about DTG-PCR. A newly invented tool by modifying the typical PCR for solving traveling salesman problem

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denaturation temperatuare gradient polymerase chain reaction by product

Denaturation temperatuare gradient-polymerase chain reaction: by-product?

Ji Youn Lee

Cell and microbial engineering laboratory

Seoul National University

let s talk about dtg pcr
Let’s talk about DTG-PCR
  • A newly invented tool by modifying the typical PCR for solving traveling salesman problem
  • More amplification of DNA strands with lower melting temperature
  • Is it True?
  • Does it operate Well?
  • Is it Worth?
it s time to study dtg pcr
It’s time to study DTG-PCR
  • Experimental part
  • Modeling and simulation part
  • Make use of the published ones

: Competitive PCR

  • Addition of the ssDNA distribution profile with temperature
experimental considerations
Experimental considerations
  • DNA strands with different Tms
    • Same length, different Tm
    • or Different length, different Tm
    • or Different length, same Tm… etc.
  • Experiments
    • Mix the strands (two or more)
    • Perform DTG-PCR in various conditions
    • Analysis: TGGE, DGGE or graduate PCR
theoretical considerations
Theoretical considerations
  • Studies up to date
    • Competitive PCR
    • Quantitative PCR
    • Internal standards and products
    • Anal Biochem (1993)
      • Quantitative PCR: Theoretical considerations with practical implications
      • Replication efficiency E (product and standard)
slide7
NAR (1996)
    • A mathematical model and a computerized simulation of PCR using complex templates
    • Probability calculation
  • PNAS (1996)
    • Efficiency of DNA replication in the PCR
  • NAR (1997)
    • A coalescent approach to the PCR
  • NAR (1998)
    • Modeling and analysis of competitive RT–PCR
slide8
JTB (1997)
    • Enzymological considerations for a theoretical description of the quantitative competitive PCR
    • Michaelis-Menten equation (enzyme kinetics)
  • JTB (1997)
    • Theoretical description of the PCR
  • JTB (1999)
    • Polymerase chain reaction: Markove process approach
  • Bioinformatics (2001)
    • Virtual PCR
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