1 / 13

Cytokine Patterns in Adults with AIDS (Kenneth A. et.al.)

Cytokine Patterns in Adults with AIDS (Kenneth A. et.al.). By Alidana Castillo Seminar 2000. Definitions:. Cytokines : proteins released in response to antigen which influences other cells. Antigen : substance that causes the production of specific antibodies.

aram
Download Presentation

Cytokine Patterns in Adults with AIDS (Kenneth A. et.al.)

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript


  1. Cytokine Patterns in Adults with AIDS(Kenneth A. et.al.) By Alidana Castillo Seminar 2000

  2. Definitions: • Cytokines: proteins released in response to antigen which influences other cells. • Antigen: substance that causes the production of specific antibodies. • Interleukins: (IL) group of proteins produced by macrophages and T cells that regulate growth and differentiation of lymphoncytes.

  3. Definitions (cont.): • Phytohemagglutinin: (PHA) a substance that triggers disease progression. • Th1 and Th2: T helper cells that produce antibodies and are enhanced by the T cells and that also contain CD4 + cells. • TNF-B: Tumor necrosis factors (Beta). • TGF-B: Transforming growth factor (Beta).

  4. Introduction: • Reports on HIV infected patients and enhanced production of IL-4 and IL-10 in response to stimulation of peripheral blood lymphocytes (PBL) with phytohemagglutinnin (PHA). • Role of Th1 and Th2 predominance pathway in HIV infection.

  5. Rationale: • Cross-sectional study of cytokine levels analyzing for various proteins in peripheral blood. • To determine if the cytokine expression in patients with AIDS depends upon the character of the stimulus.

  6. Methods: • Patients: • Used (29) men and (2) women with AIDS without active infections. • (1) woman and (4) men, normal healthy volunteers. • Serum Collection: • Seven ml of whole blood were collected and placed in silicone coated tubes (in vitro).

  7. Methods (cont.): • Used the following cytokines to observe the increased levels in AIDS patients… IL-1, IL-2, IL-3, IL-4, IL-6, IL-6SR, IL-8, IL-12, IFN-A, IFN-G, TNF-A, TNF-B, OSM, and RANTES.

  8. Data Analysis: • Researchers used Spearman correlation to analyze group-related differences in patterns of cytokine levels. • In addition, used the Wilcoxon rank sum test to compare the cytokine in phytohemagglutinin (PHA).

  9. Results (Table): • The patients were grouped by… • Age • Percentage of CD4+ cells

  10. Results (cont.): • Peripheral blood lymphocytes from AIDS patients, when stimulated in vitro, expressed low levels of cytokines tested, except TGF-B, TNF-B, IL-3, IL-4, and IL-8 when compared to normal volunteers. • For IL-2 and IL-12 it varied between the normal volunteers and AIDS patients. • As for the other cytokine expressions there was not much difference found.

  11. Results (Table): • Cytokine expression in peripheral blood lymphocyte was generally higher in AIDS patients for most of the cytokines tested.

  12. Conclusion: • There is no difference in pattern of cytokine expression in peripheral blood when compared to expression of peripheral blood lymphocytes or CD4+ enriched peripheral blood cells in the patients. • It was difficult to study transduction and cross-regulation between Th1 or Th2 cells.

  13. Conclusion (cont.): • The high levels of IL-3, IL-4, IL-8, and TNF-B in AIDS patients when compared to normal healthy volunteers were determined to be caused by chronic active disease. • It was concluded that the character of cytokine response in AIDS patients may be directly related to the stimulus caused in test systems.

More Related