Prevascularized Acellular Lung Construct for Tissue Engineering. Ryan Nagao Nov 03, 2010. Objective. Create a construct that can be connected to the host vascular system for rapid anastomosis and tissue survival for large defects. Problem to Address.
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Nov 03, 2010
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EC cell seeding
Selected for OA process
Sodium dodecyl sulfate (SDS)
Tom WJ., et al. IEEE T Med Imaging, 2008
Hematoxylin and eosin staining of fresh lung (top) and following OA process (bottom) at different magnifications (4x, 10x, 20x, 40x). All nuclei are removed from the OA process; however, an intact ECM persists.
Immunostaining against laminin (red) and fibronectin (green) with DAPI (blue) of native rat lung (left) and standard OA rat lung (right) reveals an intact ECM remains following the standard concentration OA procedure on rat lung. Scale bar = 200 µm
Immunostaining for CD-31 (green) with DAPI (blue) of fresh (A) and decell (B,C) lung following OA processing at low concentration (B) and standard concentration (C). Standard concentration removes all cellular components. Bar, 100 µm
Cresyl violet injected into the pulmonary artery of the right lung of a rat following OA processing. Note: dye expanded to all the lobes of the lung except for the accessory lobe (arrow).
Cell morphology in fibrin and PEGylated fibrin after 7 days of culture. Human MSCs in (A) 2D culture; (B) fibrin only; (C) NHS-PEG fibrin; (D) BTC-PEG fibrin; (E) SC-PEG fibrin; (F) SMB-PEG fibrin. Immunofluorescent staining for CD31 (G), VWF (H), VE-cadherin (I) and Flk-1 (J) of hMSCs embedded in BTC-PEG fibrin (as in D). Nuclear counterstain with DAPI. Bar, 10 μm (Zhang, 2010)