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Glut. 80 mM Pi. EGTA. Effect of 80 mM Pi on SR [Ca 2+ ], in the presence of 0.4 mM Mg 2+ , 100 nM Ca 2+ , 10 mM CP 5 mM ATP and 1 mM caffeine. No sparks were seen in any soln used here. 113004a. 50 mM EGTA (0 Ca). SR [Ca 2+ ] ( μ M). Glut. 20mM Pi. EGTA. SR [Ca 2+ ] ( μ M).
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Glut 80 mM Pi EGTA Effect of 80 mM Pi on SR [Ca2+], in the presence of 0.4 mM Mg2+, 100 nM Ca2+, 10 mM CP 5 mM ATP and 1 mM caffeine. No sparks were seen in any soln used here. 113004a
50 mM EGTA (0 Ca) SR [Ca2+] (μM) Glut 20mM Pi EGTA SR [Ca2+] (μM) Precipitation of Ca2+ in the SR in the presence of 20 mM cytoplasmic Pi. 113004b
80 mM Pi EGTA Glut 80 mM Pi Effect of 80 mM Pi on SR [Ca2+], in the presence of 0.4 mM Mg2+, 100 nM Ca2+, 10 mM CP 5 mM ATP and 1 mM caffeine. 113004c
20 mM Pi EGTA Glut 20 mM Pi Precipitation of Ca2+ in the SR in the presence of 20 mM cytoplasmic Pi. 113004d
120204a No precipitation after 10 min in 20 mM SO42- and 100 nM Ca2+.
