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Role of Human PapillomaVirus infection and other co factors in the aetiology of Head and Neck Cancer in Europe and India. Block Details : Total number of blocks received in RGCB –1004 Different sites from which we received the blocks are: Ambilikkai -148 Bangalore- 344 Guwahati-346

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Role of Human PapillomaVirus infection and other co factors in the aetiology of Head and Neck Cancer in Europe and India


  • Block Details :

  • Total number of blocks received in RGCB –1004

  • Different sites from which we received the blocks are:

  • Ambilikkai -148

  • Bangalore- 344

  • Guwahati-346

  • Barshi-156

  • Trivandrum -10


  • Serum transferred to RGCB -540 (RCC, Trivandrum)

  • Total number of blocks sectioned -69

  • Blocks returned back to Bangalore after sectioning: 25

  • Blocks take n to IARC: Prospective-68(Ambilikkai), Retrospective-80(Ambilikkai)

  • Guwahati -40

  • Total : 188

  • Total number of shavings taken to IARC: 116 tubes and 13 boxes


  • Oral Saline washouts :

  • Pune: 320

  • Ambilikkai:216

  • Barshi: 341

  • DNA isolated: 877


  • Head and Neck Cancer is the 6th common malignancy reported worldwide with approximately 6,50000newcases and 3,50000 deaths are reported annually

  • Cancers of the head and neck include cancers of the oral cavity, larynx, pharynx, salivary glands and nasal passage

  • The prevalence of HPV in oral cavity, larynx and hypopharynxappears to be considerably lower

  • Oral cancer subtype of HNC(85%)

  • Several types of oral cancer ,around 90% squamous cell carcinoma originating in the tissue that line mouth and lips


  • Causative factors of oral cancer:

  • Alcohol abuse, radiation exposure, tobacco use, poor nutrition and poor hygiene

  • HPV is considered to be one of the factor

  • Symptoms include lump or sore that does not heal, difficulty in swallowing, change of hoarseness in the voice


  • Human papilloma virus:

  • Papillomaviridae family, double stranded DNA virus, infects cutaneous/mucosal surfaces

  • Infects basal layer of the skin

  • Genome: 8000basepairs, divided into 3 main domains a noncoding URR, an early region with ORF,and late region encoding capsid proteins

  • E6 and E7 binds to the tumor supressor gene pRb and p53


  • Objective of the study:

  • To address the crucial still unanswered questions on HNC aetiology with particular focus on the role of HPV infection

  • To determine whether HPV interact with additional HNC risk factors in Europe and Indian population

  • To know prevalence of DNA/RNApositivity in oral specimens and also to explore novel screening stratergies that could be broadly used in clinical routine


  • Materials and Methodology:

  • Total study size 2000 cases and 2000controls

  • Cases include cancers of the oral cavity , oropharynx, hypopharynx, and larynx

  • Samples include serum, oral saline wash and formalin fixed paraffin embedded tissue


  • Methodology:

  • Serology

  • Genotyping

  • Immunohistochemistry

  • Luminexxmap technology


  • Genotyping:

  • The process of determining difference in the genetic makeup of an individual by examining the individual DNA sequence using biological assays and comparing to another individual sequence for reference

  • Luminexxmap technology for detecting 19 high risk HPV types (16,18,26,31,33,35,39,45,51,52,53,56,58,59,66,68,70,73,82)and 2 low risk hpv types(6,11)

  • The assay includes Beta globin gene to evaluate the presence and quality of extracted DNA


Outline

  • Luminex® xMAP Technology


DETECTION OF THE BEADS USING LASER


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