Extraction and Quantitation of DNA From E. coli. Experiment 6. QUIZ. Write the brief procedure for cloning the gene X from the Vibrio fischeri to the E.coli ? Write the open structure of DNA (3-4 bp ) by using followings? (no need to draw H bonds). S. Pyr. Purine. P.
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Extraction and Quantitation of DNA From E. coli
-To get rid of growth medium
Absorbance at 260 nm
DNA concentration (µg/ml) = (OD 260) x (dilution factor) x (50 µg DNA/ml)/(1 OD260 unit)
1,8 < OD 260 / OD 280 < 2, pure enough
OD 260 / OD 280 < 1,8, high protein rate