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Reminders

Reminders. Animations on blackboard & web site. CD- “Clusters of Differentiation”. WALKER BIOLOGY CLUB MEETING & GUEST SPEAKER!!. Dr. Larry Grand from N.C. State’s Plant Pathology Department will be speaking!

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Reminders

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  1. Reminders • Animations on blackboard & web site. • CD- “Clusters of Differentiation”

  2. WALKER BIOLOGY CLUB MEETING & GUEST SPEAKER!! Dr. Larry Grand from N.C. State’s Plant Pathology Department will be speaking! Come hear about the Plant Pathology Graduate Program at N.C. State, as well as graduate programs & applications in general! February 27, 2008 5:00 p.m. in Room 210 Pizza & Drinks Provided

  3. Antigen Processing & Presentation The exogenous pathway continued

  4. Cathepsins- S & L: acid proteases

  5. -Cathepsins S & L: acid proteases

  6. MHC class II compartments- (MIIC) • 2-4 hrs to travel to endosome / phagosome.

  7. MIIC

  8. What will happen if there is no infection? • What would happen if a pathogen lives inside the phagosomes of the macrophage? • Leishmania sp • Mycobacterium sp • Coxiella burnetti.

  9. Dendritic cells

  10. The two classes of MHC are differentially expressed on cells:

  11. The T cell & the TCR

  12. The TCR p. 68

  13. 3 Dimensional Structure of the a/b TCR: • CDR or HV regions

  14. p. 72

  15. CD3 Complex CD3 heterdimers Epsilon-delta Epsilon-gamma Invariant accessory chains. The TCR interacts with other proteins to make the TCR Complex: • Zeta/zeta homodimer

  16. Coreceptors function: CD4 recognizes & binds to the class II MHC. CD8 recognizes & binds to the class I MHC. Increase cell signaling event 100 fold. hinge The TCR complex requires coreceptors to be active: Fig. 3.10, p. 74, 2nd edition

  17. How does the TCR complex Interact with the MHC-antigenic-peptide?

  18. The TCR must recognize & bind to both the MHC molecule & the foreign peptide.

  19. Light & dark blue- beta chain CDR1 & CDR2 Light & dark purple- alpha chain CDR1 & CDR2. Yellow- alpha chain CDR3 Green- beta chain CDR 3 Red- HV4 of beta chain. Dark Yellow- peptide in MHC.

  20. Vb interacts with MHC-a1 & carboxy terminus of peptide. Va interacts with MHC-a2 & amino terminus of peptide. peptide a1 MHC a2

  21. MHC alpha 1 MHC alpha 2 alpha & beta CDR3 make most contact with Ag peptide.

  22. Where do CD4 & CD8 bind to the MHC?

  23. CD8 alpha binds to the a3 and a2 of the MHC class I. CD8a

  24. b-2 microglobulin

  25. TCR- beta CD8 binding is distally located from the TCR binding area. TCR alpha MHC alpha CD8 alpha& beta MHC b-2

  26. CD4 D1 binds to the b2 subunit in a hydrodphobic crevice between beta 2 & alpha 2 on the MHC class II. D1

  27. CD4 D1 binds to the b2 subunit in a hydrodphobic crevice between beta 2 & alpha 2 on the MHC class II. alpha beta D2 D1

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