MYB61. Single or Multicopy gene in Arabidopsis Thaliana ?. Research Plan. Isolate Genomic DNA. Southern Blot Analysis. Digest Genomic DNA w/ Various Restriction Enzymes. Agarose Gel Electrophoresis and Southern Transfer. Make Non-Radioactive Myb61 Probe. Hyribidize Probe to Southern Blot.
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Single or Multicopy gene in Arabidopsis Thaliana?
Isolate Genomic DNA
Southern Blot Analysis
Digest Genomic DNA w/ Various Restriction Enzymes
Agarose Gel Electrophoresis and Southern Transfer
Make Non-Radioactive Myb61 Probe
Hyribidize Probe to Southern Blot
Washes and Chemiluminescent Detection
1. To Isolate High Quality Genomic DNA from Arabidopsis
2. Determine the Quantity and Purity of the Genomic DNA
The arabidopsis information resource: http://www.arabidopsis.org/
Fundamental Steps in Isolation:
Break open cells
Extract DNA from other cellular components
Disrupts tissues and facilitates breaking of cells
Components of Reaction Buffer
Cesium Chloride Gradient
Ultraviolet Spectroscopic Analysis of Nucleic Acids