The Effects of Caffeine on Plant Germination

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Question. How will different amounts of caffeine exposure affect the growth and germination of radish seeds?. Background Information.
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The Effects of Caffeine on Plant Germination

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1. The Effects of Caffeine on Plant Germination By: Erin Chandler

2. Question How will different amounts of caffeine exposure affect the growth and germination of radish seeds?

3. Background Information #1 drug in America Caffeine is better known as trimethylxanthine The chemical formula of trimethylxanthine is C8H10N4O2 Naturally made in some plants Used in sports Caffeine and the brain Growth hormone or inhibitor? Raphannus Sativus Growing conditions Process of germination

4. Hypothesis If seeds are exposed to caffeine solutions of various concentrations, then those exposed to the highest concentration will have higher germination rates and longer root lengths then those exposed to solutions with lower caffeine concentration.

5. Materials bleach distilled water 5 beakers 200mg caffeine pill mortar and pestle radish seeds 20 Petri dishes 12 pieces of filter paper dropper gloves

6. Procedure Wear gloves while making the bleach solution. Put seeds in a bleach/water solution and rinse Label beakers 1-5 and fill beaker 1 with 100 mL of water Crush caffeine pill and put it in beaker 2 with 100 mL of water, stir Take 10 mL from beaker 2 and add to beaker 3 along with 90 mL of water, stir Take 10 mL from beaker 3 and add it to beaker 4 along with 90 mL of water, stir Take 10 mL from beaker 4 and add it to beaker 5 along with 90 mL of water, stir Label Petri dishes according to caffeine concentration and then trials 1-4 Soak filter paper in 3 mL of solution and place in appropriate Petri dish Put 10 radish seeds in each Petri dish Put Petri dishes in a dark enclosed area for 3 days and observe

7. Variables Control: seeds exposed to distilled water Independent Variable: amount of caffeine in each solution Dependent Variables: amount of seeds that germinate, and root lengths Constants: amount of solution on filter paper, temperature, number of seeds in each Petri dish, temperature of water

8. Data


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