Download Policy: Content on the Website is provided to you AS IS for your information and personal use only and may not be sold or licensed nor shared on other sites. SlideServe reserves the right to change this policy at anytime. While downloading, If for some reason you are not able to download a presentation, the publisher may have deleted the file from their server.
1. The Effects of Caffeine on Plant Germination By: Erin Chandler
2. Question How will different amounts of caffeine exposure affect the growth and germination of radish seeds?
3. Background Information #1 drug in America
Caffeine is better known as trimethylxanthine
The chemical formula of trimethylxanthine is C8H10N4O2
Naturally made in some plants
Used in sports
Caffeine and the brain
Growth hormone or inhibitor?
Process of germination
4. Hypothesis If seeds are exposed to caffeine solutions of various concentrations, then those exposed to the highest concentration will have higher germination rates and longer root lengths then those exposed to solutions with lower caffeine concentration.
5. Materials bleach
200mg caffeine pill
mortar and pestle
20 Petri dishes
12 pieces of filter paper
6. Procedure Wear gloves while making the bleach solution. Put seeds in a bleach/water solution and rinse
Label beakers 1-5 and fill beaker 1 with 100 mL of water
Crush caffeine pill and put it in beaker 2 with 100 mL of water, stir
Take 10 mL from beaker 2 and add to beaker 3 along with 90 mL of water, stir
Take 10 mL from beaker 3 and add it to beaker 4 along with 90 mL of water, stir
Take 10 mL from beaker 4 and add it to beaker 5 along with 90 mL of water, stir
Label Petri dishes according to caffeine concentration and then trials 1-4
Soak filter paper in 3 mL of solution and place in appropriate Petri dish
Put 10 radish seeds in each Petri dish
Put Petri dishes in a dark enclosed area for 3 days and observe
7. Variables Control: seeds exposed to distilled water
Independent Variable: amount of caffeine in each solution
Dependent Variables: amount of seeds that germinate, and root lengths
Constants: amount of solution on filter paper, temperature, number of seeds in each Petri dish, temperature of water