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Supplementary Figure legends Supplementary Figure 2. Phenotypic and functional characterization of PD-MSC.

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Supplementary Figure legends

Supplementary Figure 2. Phenotypic and functional characterization ofPD-MSC.

(A) Morphology and karyotype (44+XX) of PD-MSC. PD-MSC showed fibroblastic-like morphology. Magnification:x100 (B) Cytometric analysis of PD-MSC for the MSC marker CD44, CD73, CD90 and CD105, the hematopoeitic/endothelial cell marker CD34, CD31, CD45 and the MSC migration marker CD29, CD49 and the immune suppression marker CD54, CD102, CD106, CD166 and non-trophoblast marker CD9. (C) PD-MSC single cell size is average 14.3μm. (D) Population doubling level in PD-MSC. (E) ELISA assay of pro-angiogenic factor/neurotropic factor (VEGF, TGFß1) released by PD-MSC. (F) Differentiation potential of PD-MSC. Magnification:x100


B

A

CD73

CD105

CD44

CD90

CD31

CD29

CD34

CD45

Cell counts

CD54

CD166

CD49

CD106

C

D

SSEA4

HLA-DR

CD9

HLA-ABC

Fluorescence

E

F

Osteocyte

(Von kossa)

Chondrocyte

(Alcian blue)

Adipocyte

(Oil red O)

CON

PD-MSC

CON

PD-MSC


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