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Plant-Based Biopharmaceutical Production under Controlled Environments

Plant-Based Biopharmaceutical Production under Controlled Environments. Jessica Gamboa- University of Arizona Biosystems Engineering Chieri Kubota, Ryo Matsuda, Lucrecia M. Alvarez, & Guy A Cardineau. Our Eventual Goal.

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Plant-Based Biopharmaceutical Production under Controlled Environments

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  1. Plant-Based Biopharmaceutical Production under Controlled Environments Jessica Gamboa- University of Arizona Biosystems Engineering Chieri Kubota, Ryo Matsuda, Lucrecia M. Alvarez, & Guy A Cardineau

  2. Our Eventual Goal • Use plant made pharmaceuticals to produce an easily deliverable form of the vaccine for the plague (Y. pestis) • This will be done using plant-made pharmaceuticals grown under a controlled environment

  3. Plague • Plague is an infectious disease that is caused by the bacterium Yersinia pestis, it can be deadly. • The F1-V protein is used as an antigen fusion protein against plague.

  4. Experimental Goal To quantify… • plant growth • development • photosynthesis • total soluble protein concentration

  5. The tomato plants were modified to produce edible F1-V protein, a form of the vaccine for the plague • Greenhouse growth is advantageous because high production and containment is achievable

  6. Items measured in experiment • Stem growth • Leaf production • Light-saturated rate of photosynthesis in leaves • Weekly fruit yield • Total soluble-protein concentration • F1-V concentration in fruits

  7. Plants Grown in Greenhouse • ‘Durinta’ - Commercial greenhouse cultivator • ‘TA234’ - Wild Type • ‘T22.1’ - Transgenic line • ‘T3D1.2’ - Supertransformed line

  8. Plant Growth and Leaf Production • Stem Length ‘Durinta’>‘T22.1’=‘TA234’= ‘T3D1.2’ • Number of Leaves ‘Durinta’= ‘T22.1’ =‘TA234’=‘T3D1.2’

  9. Photosynthesis, Fruit Yield and Total Soluble Protein • Light-saturated rate of photosynthesis ‘Durinta’ ≥ ‘T22.1’ = ‘TA234’ = ‘T3D1.2’ • Fruit Yield ‘Durinta’ > ‘TA234’ > ‘T22.1’ =‘T3D1.2’ • Total Soluble Protein in Green Fruits ‘T22.1’ > ‘T3D1.2’ > ‘TA234’ >‘Durinta’

  10. Conclusion • Transgenic tomato plants showed a lower growth rate and lower yield, but produced more protein than ‘Durinta’, the commercial greenhouse standard. • Transgenic tomato plants are considered promising candidates for high value protein production.

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